Induction of Immune Response in Animal Model Using Recombinant Anti-NDV Vaccine
| Autor: | Amir Ghaffar Shahriari, Abdolreza Bagheri, Maziar Habibi-Pirkoohi, Alireza Afsharifar |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Newcastle Disease
Biology Biochemistry Newcastle disease Virus Epitope law.invention 03 medical and health sciences Immune system Western blot law Genetics medicine Mucosal Immunity Gene 030304 developmental biology 0303 health sciences medicine.diagnostic_test Immunogenicity Western Blot 04 agricultural and veterinary sciences biology.organism_classification Virology Recombinant Vaccine 040102 fisheries Recombinant DNA 0401 agriculture forestry and fisheries ELISA Immunogenic Response Research Article Biotechnology |
| Zdroj: | Iranian Journal of Biotechnology |
| ISSN: | 2322-2921 |
| Popis: | Background Newcastle disease is a major avian disease that causes enormous economic loss in poultry industry. There have been a number of reports on the suitability of plant-based recombinant vaccine against this disease. Fusion (F) and hemagglutinin-neuraminidase (HN) epitopes of the Newcastle disease virus (NDV) represent the major immunogenic sites for development of recombinant anti-ND vaccines in plant hosts. Objectives The main objective of this research was to evaluate the ability of a recombinant anti-ND vaccine in induction of immune responses in animal model. Materials and methods In this study, immunogenicity of recombinant fusion (F) and hemagglutinin-neuraminidase (HN) epitopes of the Newcastle disease virus (NDV) is investigated in an animal model. The corresponding genes encoding amino acids 65-81 of the F protein and 346-353 amino acids of HN were expressed in tobacco seedling using agrobacterium-mediated transformation. Expression of the foreign gene in the tobacco seedlings was investigated by a number of molecular assays including Real-Time PCR and ELISA. Transgenic plant extract was used to induce immunogenic response in animal model. Results Integration of the foreign gene in plant host genome was confirmed by polymerase chain reaction (PCR). Expression of the foreign recombinant protein was confirmed by Real-Time PCR and ELISA assays. Immunogenicity of the recombinant protein was investigated in rabbit by subcutaneous injection. Results indicated that the transgenic plant extract can induce immune responses in the host as confirmed by presence of specific antibodies in the sera in ELISA assay. Western blot assays showed that the foreign gene was actually expressed in transgenic seedlings. Conclusions The results obtained in this research provide further evidence on applicability of plant-based recombinant vaccines for protection of poultry against Newcastle disease. |
| Databáze: | OpenAIRE |
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