Identification of an HSP70-related protein associated with the centrosome from dinoflagellates to human cells
| Autor: | Eric Perret, Marie-Odile Soyer-Gobillard, J. Derancourt, Marie-Line Géraud, Michel Bornens, Mohammed Moudjou |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.drug_class
Molecular Sequence Data Protozoan Proteins Antigens Protozoan Biology Monoclonal antibody Antibodies Cell Line Polyethylene Glycols symbols.namesake Antigen medicine Animals Humans HSP70 Heat-Shock Proteins Amino Acid Sequence Microtubule nucleation Centrosome Sequence Homology Amino Acid Cell Cycle Cell Biology Golgi apparatus Cell biology Polyclonal antibodies Chaperone (protein) symbols biology.protein Dinoflagellida Antibody |
| Zdroj: | Journal of cell science. 108 |
| ISSN: | 0021-9533 |
| Popis: | The monoclonal antibody CTR210 raised against isolated human centrosomes strongly decorates the centrosome and more weakly a domain congruent with the Golgi apparatus in several animal cells (HeLa, 3T3, CHO, PtK2). Both decorations resist Triton extraction in conditions which totally extract the Golgi apparatus, as judged by galactosyltransferase decoration. A 67 kDa centrosomal antigen can be demonstrated in human cells with this antibody. CTR210 also decorates the centrosome or associated structures in several systems, including unicellular eukaryotes such as dinoflagellates or ciliates. A 72 kDa antigen has been identified and purified from the dinoflagellate C. cohnii and its NH2-terminal sequence partially established. It shows a close homology with HSP70 proteins. The possibility that the 72 kDa antigen belongs to this chaperone family was further supported using a mAb reacting, in most species, with HSP70. A polyclonal antibody raised against the 72 kDa antigen from C. cohnii decorates the centrosome in human cells and reacts with the CTR210 centrosomal 67 kDa antigen. These results suggest that specific chaperone proteins are associated with the centrosome in eukaryotic cells. The centrosomal chaperones could participate in the microtubule nucleation reaction or in the process of centrosome assembly. |
| Databáze: | OpenAIRE |
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