Luminometric Nanoparticle-Based Assay for High Sensitivity Detection of β-Amyloid Aggregation
| Autor: | Sari Pihlasalo, Pia Vuorela, Roope Huttunen, Karthik Chary, Maria Virtamo, Gerda Brunhofer-Bolzer, Takahiro Deguchi, Francisco R. López-Picón, Adyary Fallarero, Harri Härmä, Jenna Jacobino |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Analytical chemistry Nanoparticle chemistry.chemical_element Peptide macromolecular substances Protein aggregation 010402 general chemistry Microscopy Atomic Force 01 natural sciences 7. Clean energy Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Protein Aggregates Adsorption Europium Microscopy Fluorescence Resonance Energy Transfer ta116 Fluorescent Dyes chemistry.chemical_classification Benzophenanthridines Amyloid beta-Peptides Chemistry Atomic force microscopy ta1182 Peptide Fragments 0104 chemical sciences 030104 developmental biology Biophysics Nanoparticles Polystyrenes Thioflavin Rifampin |
| Zdroj: | Analytical Chemistry. 89(4):2398-2404 |
| ISSN: | 0003-2700 |
| Popis: | A nanoparticle-based assay utilizing time-resolved luminescence resonance energy transfer (TR-LRET) was developed for the detection of β-amyloid aggregation. The assay is based on the competitive adsorption of the sample and the acceptor-labeled protein to donor europium(III) polystyrene nanoparticles. The performance of the assay was demonstrated by following the fibrillization of β-amyloid peptide 1–42 (Aβ42) as a function of time and by comparing to the reference methods atomic force microscopy (AFM) and thioflavin T (ThT) assay. The fibrillization leads to reduced adsorption of Aβ42 to the nanoparticles increasing the TR-LRET signal. The investigated methods detected fibril formation with equal sensitivities. Eight potential fibrillization inhibitor compounds reported in the literature were tested and the results obtained with each method were compared. It was shown with AFM imaging that the inhibition of fibril formation was not complete with any of the compounds. The developed TR-LRET nanoparticle a... |
| Databáze: | OpenAIRE |
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