Endocytosis of GM-CSF receptor β is essential for signal transduction regulating mesothelial-macrophage transition
| Autor: | Sándor Katz, Anna L. Kiss, Nikolett Dóczi, Viktória Zsiros |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Receptor recycling Endosome media_common.quotation_subject Endocytic cycle Caveolae Endocytosis Cytokine Receptor Common beta Subunit Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Transforming Growth Factor beta STAT5 Transcription Factor Animals Phosphorylation Internalization Receptor Molecular Biology media_common Inflammation Chemistry Macrophages GM-CSF Receptor Hydrazones Cell Biology Janus Kinase 2 Rats Cell biology Disease Models Animal 030104 developmental biology 030220 oncology & carcinogenesis Cell Transdifferentiation Signal transduction Signal Transduction |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1866:1450-1462 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/j.bbamcr.2019.06.005 |
| Popis: | During Freund's adjuvant induced inflammation rat mesenteric mesothelial cells transdifferentiate into mesenchymal cell. They express macrophage markers, inflammatory cytokines (TGF-β, TNFα, IL-6), and specific receptors. When primary mesenteric cultures were treated with GM-CSF and/or TGF-β (in vitro), similar phenotypic and biological changes were induced. It seemed likely that GM-CSF receptor-ligand complex should be internalized to initiate mesothelial-macrophage transition. To follow the intracellular route of GM-CSF receptor β, we co-localized this receptor with various endocytic markers (Cav-1, EEA1, Rab7, and Rab11a), and carried out detailed immunocytochemical, statistical and biochemical analyses. Since STAT5 is one of the downstream element of GM-CSF signaling, we followed the expression and phosphorylation level of this transcription factor. Our results showed that in mesenteric mesothelial cells GM-CSF receptor β is internalized by caveolae, delivered into early endosomes where the signaling events occur, STAT5A is phosphorylated by JAK2, and then translocated into the nucleus. When dynamin-dependent endocytosis of GM-CSFR β is inhibited by dynasore, phosphorylation of STAT5A is not occurred, confirming, that the internalization of receptor β is indispensable for signal transduction. At the early time of inflammation a significant receptor recycling can be found to the plasma membrane. Later (day 8) the receptor is delivered into late endosomes, indicating that its degradation has already started, and the regeneration of mesothelial cells can start. All of these data strongly support that the internalization of GM-CSF receptor β is required and essential for signal transduction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect