Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration
| Autor: | Masayuki Yanagisawa, Miho Ogawa, Koh-ei Toyoshima, Kyosuke Asakawa, JingJing Tong, Akio Sato, Takashi Tsuji, Makoto Takeo, Tarou Irié |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Organ induction
Science Integrin CD34 Mice Nude Tenascin Mice Transgenic Regenerative medicine Article Mice Regeneration Animals Humans Adult stem cells Mice Inbred BALB C Multidisciplinary biology integumentary system Chemistry Multipotent Stem Cells Regeneration (biology) Embryogenesis Epithelial Cells Cell biology biology.protein Medicine Stem cell Hair Follicle Skin stem cells |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | In mammals, organ induction occurs only during embryonic development except for hair follicles (HFs). However, HF-resident epithelial stem cells (HFSCs), which are responsible for repetitive HF regeneration, are not fully characterized. Here, we establish in vitro culture systems that are capable of controlling the ability of HFSCs to regenerate HFs. Based on a method that precisely controlled the number of HFs for regeneration, functional analysis revealed that CD34/CD49f/integrin β5 (Itgβ5)-triple-positive (CD34+/CD49f+/Itgβ5+) cells have multipotency and functional significance for continual hair regeneration. In native HFs, these cells reside in the uppermost area of the bulge region, which is surrounded by tenascin in mice and humans. This study unveils the subpopulation of HFSCs responsible for long-term hair cycling of HFs regenerated from bioengineered HF germ, suggesting the presence of functional heterogeneity among bulge HFSCs and the utility of our culture system to achieve HF regenerative therapy. |
| Databáze: | OpenAIRE |
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