Inhibitor of Growth 4 (ING4) is a positive regulator of rRNA synthesis
| Autor: | Tomohiro Kimura, Natsumi Sakata, Ryutaro Shirakawa, Duc Anh Trinh, Hisanori Horiuchi, Kota Goto |
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| Rok vydání: | 2018 |
| Předmět: |
Transcription
Genetic Nucleolus Regulator lcsh:Medicine Ribosome biogenesis Cell Cycle Proteins DNA Ribosomal Article Histones Stress signalling Cell Line Tumor Histone post-translational modifications Humans lcsh:Science Promoter Regions Genetic Gene Cell Nucleus Homeodomain Proteins Multidisciplinary biology Tumor Suppressor Proteins lcsh:R Wild type Acetylation RRNA transcription Cell biology Histone RNA Ribosomal biology.protein Homeobox RNA lcsh:Q HeLa Cells |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Ribosome biogenesis is essential for maintaining basic cellular activities although its mechanism is not fully understood. Inhibitor of growth 4 (ING4) is a member of ING family while its cellular functions remain controversial. Here, we identified several nucleolar proteins as novel ING4 interacting proteins. ING4 localized in the nucleus with strong accumulation in the nucleolus through its plant homeodomain, which is known to interact with histone trimethylated H3K4, commonly present in the promoter of active genes. ING4 deficient cells exhibited slower proliferation and the alteration in nucleolar structure with reduced rRNA transcription, which was rescued by exogenous expression of GFP-ING4 to the similar levels of wild type cells. In the ING4 deficient cells, histone H3K9 acetylation and the key rRNA transcription factor UBF at the promoter of rDNA were reduced, both of which were also recovered by exogenous GFP-ING4 expression. Thus, ING4 could positively regulate rRNA transcription through modulation of histone modifications at the rDNA promoter. |
| Databáze: | OpenAIRE |
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