MassARRAY Spectrometry Is More Sensitive than PreTect HPV-Proofer and Consensus PCR for Type-Specific Detection of High-Risk Oncogenic Human Papillomavirus Genotypes in Cervical Cancer
| Autor: | Sam Ratnam, Adrian Lear, Dhananjaya Saranath, Maqsood Siddiqi, Partha Basu, Rameshwar N. K. Bamezai, Puneet Chandna |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Adult
Microbiology (medical) Genotype Consensus PCR Uterine Cervical Neoplasms Biology Sensitivity and Specificity Virology medicine Humans Human papillomavirus Papillomaviridae Cervical cancer Hpv types Coinfection Spectrum Analysis Papillomavirus Infections Type specific Middle Aged medicine.disease Multiple infections Molecular Diagnostic Techniques Female Kappa |
| Zdroj: | Journal of Clinical Microbiology. 49:3537-3544 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.00354-11 |
| Popis: | Type-specific detection of human papillomavirus (HPV) is indicated for better risk stratification and clinical management of women testing positive for HPV and for epidemiologic surveillance. MassARRAY spectrometry (MassARRAY; Sequenom) is a novel method for type-specific detection of 15 high-risk oncogenic HPV types: HPV-16, -18, -31, -33, -35, -39, -45, -51, -52, -56, -58, -59, -66, -68, and -73. PreTect HPV-Proofer (Proofer; Norchip) is a type-specific assay that detects E6/E7 mRNA from five high-risk oncogenic HPV types: HPV-16, -18, -31, -33, and -45. The performance of these tests for type-specific identification of HPV was assessed with cervical specimens from 192 cases of cervical cancer in comparison with consensus MY09/MY11 PCR followed by nucleotide sequencing (consensus PCR). The overall HPV detection rates were 94.8% (95% confidence interval [CI], 91.7, 97.9), 83.3% (95% CI, 78.1, 88.5), and 86.5% (95% CI, 81.7, 91.3) for MassARRAY, Proofer, and consensus PCR, respectively. All tests were negative in six (3.1%) of the 192 cases. Considering only the specimens that contained at least one of the five types targeted by Proofer, the detection rates were 96.6%, 91.4%, and 86.9% for MassARRAY, Proofer, and consensus PCR, respectively. MassARRAY detected multiple infections in 14.1%, Proofer detected multiple infections in 3.6%, and consensus PCR failed to detect any multiple infections. The agreement was highest at 86.0% (kappa = 0.76) between MassARRAY and Proofer and lowest at 81.8% (kappa = 0.69) between Proofer and consensus PCR. In conclusion, MassARRAY is a highly sensitive and accurate method for type-specific detection of oncogenic HPV in cervical cancer, with Proofer showing impressive performance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|