The Open Reading Frame 2 Product of Cacao Swollen Shoot Badnavirus Is a Nucleic Acid-Binding Protein
| Autor: | Mireille Jacquemond, Emmanuel Jacquot, L. S. Hagen, Pierre Yot |
|---|---|
| Přispěvatelé: | Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 1996 |
| Předmět: |
0106 biological sciences
Molecular Sequence Data medicine.disease_cause 01 natural sciences Open Reading Frames Viral Proteins 03 medical and health sciences Virology medicine Amino Acid Sequence Badnavirus Escherichia coli Sequence Deletion 030304 developmental biology Alanine chemistry.chemical_classification Cacao 0303 health sciences biology RNA-Binding Proteins RNA biology.organism_classification Molecular biology [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy 3. Good health Amino acid DNA-Binding Proteins Molecular Weight Open reading frame Biochemistry chemistry DNA Viral [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Nucleic acid RNA Viral Cacao swollen-shoot virus 010606 plant biology & botany |
| Zdroj: | Virology Virology, Elsevier, 1996, 225, pp.191-195. ⟨10.1006/viro.1996.0587⟩ |
| ISSN: | 0042-6822 1096-0341 |
| DOI: | 10.1006/viro.1996.0587 |
| Popis: | International audience; The function of the open reading frame 2 product (p2) of cacao swollen shoot virus (CSSV) and of other badnaviruses is not yet determined. Their carboxyl-termini are lysine and proline rich and also contain alanine residues, amino acids present at the C-termini of histone-like proteins. Full-length CSSV p2 (132 amino acids) or versions truncated at the C-terminus (128, 113, 103, or 101 amino acids) were expressed in Escherichia coli and partially purified. When assayed in nucleic acid-binding tests, p2 was able to interact with CSSV and other double-stranded DNAs and with CSSV and other single-stranded RNA transcripts in a sequence-nonspecific manner. Moreover, this binding activity was progressively lost as the C-terminus was gradually deleted. |
| Databáze: | OpenAIRE |
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