A day in the life of mitochondria reveals shifting workloads
| Autor: | John Mitrofanis, Bill T. Ferrara, Jaimie Hoh Kam, Glen Jeffery, Tobias W. Weinrich, Elinor Thompson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Transcription Genetic lcsh:Medicine Mitochondrion Biology Article 03 medical and health sciences QH301 0302 clinical medicine Adenosine Triphosphate Respiration Animals Glycolysis Atp production lcsh:Science QH426 Differential transcription Morning Multidisciplinary lcsh:R Metabolism Cellular neuroscience Cell biology Mitochondria 030104 developmental biology Optical stimulation Protein Biosynthesis lcsh:Q Drosophila Energy Metabolism 030217 neurology & neurosurgery Biomarkers |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-8 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Mitochondria provide energy for cellular function. We examine daily changing patterns of mitochondrial function and metabolism in Drosophila in vivo in terms of their complex (I-IV) activity, ATP production, glycolysis, and whole fly respiration in the morning, afternoon and night. Complex activity and respiration showed significant and unexpected variation, peaking in the afternoon. However, ATP levels by contrast are >40% greater in the morning and lowest at night when glycolysis peaks. Complex activity modulation was at the protein level with no evidence for differential transcription over the day. Timing differences between increased ATP production and peaks of complex activity may result from more efficient ATP production early in the day leaving complex activity with spare capacity. Optical stimulation of mitochondria is only possible in the mornings when there is such spare capacity. These results provide first evidence of shifts in cellular energy capacity at the organism level. Understanding their translation may be significant to the chosen timing of energy demanding interventions to improve function and health. |
| Databáze: | OpenAIRE |
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