Isolation of biosynthesis related transcripts of 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside from Fallopia multiflora by suppression subtractive hybridization
| Autor: | Xiaoze Li, Kuanpeng Zhu, Zhong-Yu Liu, Wei Zhao, Di Lu, Shujing Sheng, Shu-jin Zhao, Yan Yao |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Genetics
Expressed sequence tag biology gene expression difference 2 3 5 4'-tetrahydroxy stilbene-2-O-β-D-glucooside Plant Science biology.organism_classification Molecular biology lcsh:QK1-989 Transcriptome Rapid amplification of cDNA ends Fallopia multiflora Suppression subtractive hybridization lcsh:Botany Gene expression KEGG Gene transcriptome |
| Zdroj: | Acta Societatis Botanicorum Poloniae, Vol 83, Iss 2, Pp 147-157 (2014) |
| ISSN: | 2083-9480 |
| Popis: | 2,3,5,4'-tetrahydroxy stilbene-2-O-ß-D-glucoside (THSG) exerts multiple pharmacodynamic actions, found in Fallopia multiflora, but the biosynthesis pathway of THSG is still unclear. To clear this ambiguity, we constructed suppression subtractive hybridization (SSH) libraries to screen the genes involved in THSG biosynthesis from two F. multiflora varieties, which vary significantly in THSG content. Twelve non-redundant differentially expressed sequence tags were obtained and the full lengths of 4 unreported fragments were amplified by rapid amplification of cDNA ends. We totally got 7 full-length transcripts, and all of them were aligned to the transcriptome and digital gene expression tag profiling database of four F. multiflora tissues (root, stem and leaf from Deqing F. multiflora and another root from Chongqing F. multiflora; data unpublished) using local BLAST. The results showed that there was a significant, organ specific difference in the expression of fragments and full-length sequences. All the sequences were annotated by aligning to nucleotide and protein databases. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that THSG biosynthesis was correlated with multiple life activities. |
| Databáze: | OpenAIRE |
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