Early effects of high-fat diet on neurovascular function and focal ischemic brain injury

Autor: Weiguo Li, Victor V. Lima, Rita C. Tostes, Adviye Ergul, Darrell W. Brann, Dhruv Chawla, Roshini Prakash, Quanguang Zhang, Jessica A. Filosa, Sean P. Didion, Wenting Du
Rok vydání: 2013
Předmět:
Male
medicine.medical_specialty
Physiology
Ischemia
Hyperemia
Diet
High-Fat
N-Acetylglucosaminyltransferases
Brain Ischemia
Brain ischemia
Arteriole
Physiology (medical)
Internal medicine
medicine.artery
In Situ Nick-End Labeling
medicine
Basilar artery
Animals
Insulin
Obesity
cardiovascular diseases
Rats
Wistar
Stroke
Triglycerides
Cerebral Hemorrhage
Microscopy
Video
business.industry
food and beverages
Brain
nutritional and metabolic diseases
Infarction
Middle Cerebral Artery
medicine.disease
Neurovascular bundle
Rats
Obesity
Diabetes and Energy Homeostasis
Arterioles
Cerebrovascular Disorders
DOENÇAS CARDIOVASCULARES
Cholesterol
Blood pressure
Basilar Artery
Cerebrovascular Circulation
Anesthesia
Cardiology
lipids (amino acids
peptides
and proteins)
business
Dyslipidemia
Muscle Contraction
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1522-1490
0363-6119
Popis: Obesity is a risk factor for stroke, but the early effects of high-fat diet (HFD) on neurovascular function and ischemic stroke outcomes remain unclear. The goal of this study was to test the hypotheses that HFD beginning early in life 1) impairs neurovascular coupling, 2) causes cerebrovascular dysfunction, and 3) worsens short-term outcomes after cerebral ischemia. Functional hyperemia and parenchymal arteriole (PA) reactivity were measured in rats after 8 wk of HFD. The effect of HFD on basilar artery function after middle cerebral artery occlusion (MCAO) and associated O-GlcNAcylation were assessed. Neuronal cell death, infarct size, hemorrhagic transformation (HT) frequency/severity, and neurological deficit were evaluated after global ischemia and transient MCAO. HFD caused a 10% increase in body weight and doubled adiposity without a change in lipid profile, blood glucose, and blood pressure. Functional hyperemia and PA relaxation were decreased with HFD. Basilar arteries from stroked HFD rats were more sensitive to contractile factors, and acetylcholine-mediated relaxation was impaired. Vascular O-GlcNAcylated protein content was increased with HFD. This group also showed greater mortality rate, infarct volume, HT occurrence rate, and HT severity and poor functional outcome compared with the control diet group. These results indicate that HFD negatively affects neurovascular coupling and cerebrovascular function even in the absence of dyslipidemia. These early cerebrovascular changes may be the cause of greater cerebral injury and poor outcomes of stroke in these animals.
Databáze: OpenAIRE
Externí odkaz: