Comparative stability-indicating chromatographic methods for determination of 4-hexylresorcinol in pharmaceutical formulation and shrimps
| Autor: | Rafeek F. Shokry, Lories I. Bebawy, Mohamed R. Elghobashy, Samah S. Abbas |
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| Rok vydání: | 2017 |
| Předmět: |
Drug Compounding
Clinical Biochemistry Hexylresorcinol Pharmaceutical Science Pharmaceutical formulation 01 natural sciences High-performance liquid chromatography Analytical Chemistry 0404 agricultural biotechnology Drug Stability Tandem Mass Spectrometry Crustacea Drug Discovery Stability indicating Animals Photodegradation Spectroscopy Chromatography High Pressure Liquid 4-Hexylresorcinol Active ingredient Chromatography Chemistry 010401 analytical chemistry 04 agricultural and veterinary sciences 040401 food science 0104 chemical sciences Gradient elution Degradation (geology) Chromatography Liquid |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 145 |
| ISSN: | 1873-264X |
| Popis: | Three chromatographic stability-indicating methods were developed for determination of 4-hexylresorcinol in pure form and in a pharmaceutical formulation. Method A was based on a gradient elution liquid chromatographic HPLC determination of 4-hexylresorcinol, its related impurities and in presence of its degradation products. UPLC-MS/MS (Method B) was described for determination of the cited drug in presence of its degradation products. Method C was a thin- layer chromatography (TLC)-densitometry method for the separation and determination of the active ingredient, one of its related impurities and in presence of its degradation products. The mechanism of alkali, oxidative and photodegradation of 4-hexylresorcinol was studied according to ICH guidelines. The degradation products were characterized by the LC-MS/MS method. Methods A and B were applicable for determination of 4-hexylresorcinol residues in shrimp meat. The studied drug was easily degraded in alkali medium giving toxic compounds. The results obtained by the proposed methods were statistically analyzed and compared with those obtained by applying a reported method. |
| Databáze: | OpenAIRE |
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