Physical map of SeMNPV baculovirus DNA: An AcMNPV genomic variant
| Autor: | S. E. Brown, D.L. Knudson, James E. Maruniak |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Genes
Viral viruses EcoRI Insect Viruses Genome SmaI Viral Proteins Virology Polyhedrin Genome size Repetitive Sequences Nucleic Acid Viral Structural Proteins Genetics Base Sequence biology fungi Genetic Variation DNA Restriction Enzymes biochemical phenomena metabolism and nutrition Occlusion Body Matrix Proteins Molecular biology Molecular Weight Restriction site Restriction enzyme DNA Viral biology.protein BamHI |
| Zdroj: | Virology. 136:235-240 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(84)90262-9 |
| Popis: | A physical map of the plaque-purified SeMNPV-25 baculovirus DNA was constructed with HindIII, EcoRI, PstI, SstII, BstEII, BamHI, KpnI, and SmaI by multiple-enzyme digestion and DNA-DNA hybridization. The orientation of the physical map was to the AcMNPV polyhedrin gene, and a total of 104 restriction sites were ordered. The genome size was 131.89 kilobase pairs (87.05 × 106 Da). The physical maps of SeMNPV-25 DNA and AcMNPV-E2 DNA were compared, and they were similar except in four regions. Since the differences between the physical maps of SeMNPV-E2 and AcMNPV-E2 were reconciled, the BstEII and SstII fragments for AcMNPV-E2 were also ordered. In addition, reiterated sequences in the SeMNPV-25 genome were identified and located on the physical map. |
| Databáze: | OpenAIRE |
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