High-throughput, simultaneous quantitation of hemoglobin adducts of acrylamide, glycidamide, and ethylene oxide using UHPLC-MS/MS
| Autor: | Chui Tse, Min Yang, Hubert W. Vesper, Tunde Frame |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Ethylene Oxide Analyte Coefficient of variation Clinical Biochemistry Population 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Hemoglobins Young Adult 0302 clinical medicine Valine Limit of Detection Tandem Mass Spectrometry Humans 030212 general & internal medicine education Chromatography High Pressure Liquid Aged Detection limit education.field_of_study Acrylamide Chromatography Chemistry 010401 analytical chemistry Reproducibility of Results Cell Biology General Medicine Environmental Exposure Middle Aged 0104 chemical sciences High-Throughput Screening Assays Standard addition Linear Models Epoxy Compounds Hemoglobin |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1086 |
| ISSN: | 1873-376X |
| Popis: | Ethylene oxide (EO), acrylamide (AA) and glycidamide (GA) exposures are associated with mammary tumors in animals. Currently available information about human exposure to these chemicals is limited creating the need for analytical methods to assess their exposure. We developed a sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to simultaneously quantitate hemoglobin (Hb) N-terminal valine adducts of AA (HbAA), GA (HbGA), and EO (HbEO) using modified Edman reaction. The limits of detection of this method were 3.9, 4.9 and 12.9 in pmol/g Hb for HbAA, HbGA and HbEO, respectively. The among-day and within-day precision for all analytes determined with three levels of quality control pools ranged from 2.2–13.0% in percent coefficient of variation (%CV). The accuracy determined by standard addition was between 94 and 111% among all analytes. The median HbAA, HbGA and HbEO values in 34 self-reported non-smokers were 64.9, 45.3 and 113.6 pmol/g Hb and in 70 self-reported smokers were 127.8, 69.6 and 237.1 pmol/g Hb, respectively. HbAA, HbGA, and HbEO were detectable in all samples suggesting that the described method is suitable for measuring hemoglobin adducts of AA, GA and EO in the general population. This high throughput method can process 148 samples in 8 h. The HbEO/HbGA ratio appears independent of the HbAA levels in non-smokers and decreases with increasing HbAA concentration in smokers. This new method is suitable for measuring human exposure to AA, GA and EO and can provide further insight into the metabolism of these chemicals in humans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect