Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/- mice
| Autor: | Per Fogelstrand, Jan Borén, Farina Christopher John, Lars Glise, Ruiz Stacey, Ingrid Yao Mattisson, Jan Nilsson, Eva Bengtsson, Pontus Dunér |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Apolipoprotein B Science Fusion Proteins bcr-abl Cardiology Diseases 030204 cardiovascular system & hematology Article Mice 03 medical and health sciences 0302 clinical medicine Immune system Malondialdehyde Malaria Vaccines Animals Medicine Peptide sequence Autoantibodies Multidisciplinary biology business.industry Autoantibody Atherosclerosis Fusion protein Peptide Fragments Lipoproteins LDL 030104 developmental biology Immunization Immunoglobulin G Apolipoprotein B-100 Monoclonal Immunology biology.protein Female Antibody business |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Atherosclerotic plaques are characterized by an accumulation and subsequent oxidation of LDL, resulting in adaptive immune responses against formed or exposed neoepitopes of the LDL particle. Autoantibodies against native p210, the 3136–3155 amino acid sequence of the LDL protein apolipoprotein B-100 (apoB100) are common in humans and have been associated with less severe atherosclerosis and decreased risk for cardiovascular events in clinical studies. However, whether apoB100 native p210 autoantibodies play a functional role in atherosclerosis is not known. In the present study we immunized apoE-/- mice with p210-PADRE peptide to induce an antibody response against native p210. We also injected mice with murine monoclonal IgG against native p210. Control groups were immunized with PADRE peptide alone or with control murine monoclonal IgG. Immunization with p210-PADRE induced an IgG1 antibody response against p210 that was associated with reduced atherosclerotic plaque formation in the aorta and reduced MDA-LDL content in the lesions. Treatment with monoclonal p210 IgG produced a similar reduction in atherosclerosis as immunization with p210-PADRE. Our findings support an atheroprotective role of antibodies against the apoB100 native p210 and suggest that vaccines that induce the expression of native p210 IgG represent a potential therapeutic strategy for lowering cardiovascular risk. |
| Databáze: | OpenAIRE |
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