Ion chromatography for the precise analysis of chloride and sodium in sweat for the diagnosis of cystic fibrosis
| Autor: | J. Doorn, B. L. Rottier, W. H. A. de Jong, Ido P. Kema, A. M. Mulder, Twan T R Storteboom |
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| Přispěvatelé: | Guided Treatment in Optimal Selected Cancer Patients (GUTS), Lifestyle Medicine (LM) |
| Rok vydání: | 2014 |
| Předmět: |
Cystic Fibrosis
Sodium Clinical Biochemistry Ion chromatography chemistry.chemical_element sweattest Mass spectrometry High-performance liquid chromatography Chloride Chlorides External quality assessment medicine Humans Sweat CONDUCTIVITY Chromatography High Pressure Liquid ion chromatography PANCREAS Detection limit Reproducibility Chromatography ANALYZER General Medicine PERFORMANCE Chromatography Ion Exchange chemistry medicine.drug |
| Zdroj: | Annals of Clinical Biochemistry, 52(4), 421-427. SAGE Publications Inc. |
| ISSN: | 1758-1001 0004-5632 |
| Popis: | BackgroundMeasurement of chloride in sweat is an essential part of the diagnostic algorithm for cystic fibrosis. The lack in sensitivity and reproducibility of current methods led us to develop an ion chromatography/high-performance liquid chromatography (IC/HPLC) method, suitable for the analysis of both chloride and sodium in small volumes of sweat.MethodsPrecision, linearity and limit of detection of an in-house developed IC/HPLC method were established. Method comparison between the newly developed IC/HPLC method and the traditional Chlorocounter was performed, and trueness was determined using Passing Bablok method comparison with external quality assurance material (Royal College of Pathologists of Australasia).ResultsPrecision and linearity fulfill criteria as established by UK guidelines are comparable with inductively coupled plasma-mass spectrometry methods. Passing Bablok analysis demonstrated excellent correlation between IC/HPLC measurements and external quality assessment target values, for both chloride and sodium. With a limit of quantitation of 0.95 mmol/L, our method is suitable for the analysis of small amounts of sweat and can thus be used in combination with the Macroduct collection system.ConclusionsAlthough a chromatographic application results in a somewhat more expensive test compared to a Chlorocounter test, more accurate measurements are achieved. In addition, simultaneous measurements of sodium concentrations will result in better detection of false positives, less test repeating and thus faster and more accurate and effective diagnosis. The described IC/HPLC method, therefore, provides a precise, relatively cheap and easy-to-handle application for the analysis of both chloride and sodium in sweat. |
| Databáze: | OpenAIRE |
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