Programmable high voltage CMOS chips for particle-based high-density combinatorial peptide synthesis
| Autor: | Frank Breitling, Felix Löffler, Alexander Nesterov, U. Trunk, F. Painke, Thomas Felgenhauer, Ines Block, Kai König, Klaus Leibe, Gloria Torralba, Simon Fernandez, Volker Stadler, Volker Lindenstruth, Christopher Schirwitz, F. R. Bischoff, Michael Hausmann, J. Wagner |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
0303 health sciences
Pixel Metals and Alloys Nanotechnology High voltage 02 engineering and technology 021001 nanoscience & nanotechnology Condensed Matter Physics Chip Surfaces Coatings and Films Electronic Optical and Magnetic Materials 03 medical and health sciences CMOS Memory cell Electric field Electrode Materials Chemistry Electrical and Electronic Engineering 0210 nano-technology Instrumentation 030304 developmental biology Voltage |
| Zdroj: | Sensors and Actuators B: Chemical Sensors and Actuators B: Chemical; Vol 147 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2009.12.039 |
| Popis: | We built high voltage complementary metal oxide semiconductor (CMOS) chips that generate electrical fields on their surface, such that electrically charged microparticles (diameter 10–20 μm on average) can be addressed on distinct pixel electrodes according to arbitrary field patterns. Each pixel contains a memory cell in canonical low-voltage CMOS-technology controlling a high voltage (30–100 V) potential area on the top metal layer. Particle transfer with minimal contaminations in less than 10 s for a complete chip was observed for pixels of 100 μm × 100 μm down to 65 μm × 65 μm. This allows a new way to create surface modifications on top of CMOS chips without need for additional masks or stamps. Using suitable particles, a chemically modified chip surface, and compatible chemistry, this method can be utilized for self-aligned high-density biopolymer arrays, e.g., peptide arrays. Transfer of microparticles loaded with amino acids for combinatorial peptide synthesis is demonstrated. Successful synthesis of different peptides (octamers) was proven by immunostaining. Based on results obtained by a chip containing pixel areas of different characteristics, a chip for biological applications with 16,384 pixels (10,000 pixel/cm 2 ) was built. Good homogeneity of peptide synthesis over the chip area was verified by immunostaining. |
| Databáze: | OpenAIRE |
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