ARID3A and ARID3B induce stem promoting pathways in ovarian cancer cells
| Autor: | Paige Dausinas, Sridhar Rao, Kirthi Pulakanti, Richard Dahl, Karen D. Cowden Dahl, Jennifer M. Cole |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Programmed cell death Biology Article 03 medical and health sciences 0302 clinical medicine Cancer stem cell Cell Line Tumor GLI2 Genetics medicine Humans Gene Ovarian Neoplasms B-Lymphocytes Base Sequence Cancer General Medicine medicine.disease Phenotype Cell biology DNA-Binding Proteins 030104 developmental biology 030220 oncology & carcinogenesis Cancer cell Neoplastic Stem Cells Female Stem cell Transcription Factors |
| Zdroj: | Gene |
| ISSN: | 0378-1119 |
| Popis: | ARID3A and ARID3B are paralogs from the AT-Rich interactive Domain (ARID) family. ARID3A and ARID3B associate to regulate genes in B-cells and cancer. We were the first to demonstrate that ARID3B regulates stem cell genes and promotes the cancer stem cell phenotype. Importantly, different knockout phenotypes in mice and distinct patterns of expression in adult animals suggests that ARID3A and ARID3B may have unique functions. In addition, high levels of ARID3B but not ARID3A induce cell death. Our goal was to express ARID3A, ARID3B, or both genes at a moderate level (as can be observed in cancer) and then identify ARID3 regulated genes. We transduced ovarian cancer cells with ARID3A-GFP, ARID3B-RFP, or both. RNA-sequencing was conducted. ARID3A and ARID3B regulated nearly identical sets of genes. Few genes ( |
| Databáze: | OpenAIRE |
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