A Novel Secreted Protein Toxin from the Insect Pathogenic Bacterium Xenorhabdus nematophila
| Autor: | Anh T. Cao, Eric R. Hines, Peter D. East, Raymond J. Akhurst, Susan E. Brown |
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| Rok vydání: | 2004 |
| Předmět: |
Insecta
Time Factors Blotting Western Molecular Sequence Data Biology Helicoverpa armigera medicine.disease_cause Biochemistry Xenorhabdus Microbiology Genomic island Escherichia coli medicine Animals Genomic library Amino Acid Sequence Molecular Biology Pathogen Gene Library Toxins Biological Base Sequence Dose-Response Relationship Drug Toxin fungi Midgut Cell Biology Cosmids biology.organism_classification Recombinant Proteins Galleria mellonella Larva Biological Assay Electrophoresis Polyacrylamide Gel Additions and Corrections Photorhabdus Software Bacteria |
| Zdroj: | Journal of Biological Chemistry. 279:14595-14601 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m309859200 |
| Popis: | The bacterium Xenorhabdus nematophila is an insect pathogen that produces several proteins that enable it to kill insects. Screening of a cosmid library constructed from X. nematophila strain A24 identified a gene that encoded a novel protein that was toxic to insects. The 42-kDa protein encoded by the toxin gene was expressed and purified from a recombinant system, and was shown to kill the larvae of insects such as Galleria mellonella and Helicoverpa armigera when injected at doses of around 30-40 ng/g larvae. Sequencing and bioinformatic analysis suggested that the toxin was a novel protein, and that it was likely to be part of a genomic island involved in pathogenicity. When the native bacteria were grown under laboratory conditions, a soluble form of the 42-kDa toxin was secreted only by bacteria in the phase II state. Preliminary histological analysis of larvae injected with recombinant protein suggested that the toxin primarily acted on the midgut of the insect. Finally, some of the common strategies used by the bacterial pathogens of insects, animals, and plants are discussed. |
| Databáze: | OpenAIRE |
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