The A312L 5'-UTR of Chlorella virus PBCV-1 is a translational enhancer in Arabidopsis thaliana
| Autor: | Deane L. Falcone, Michael V. Graves, Phuong-son Nguyen |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cancer Research
Five prime untranslated region Genes Viral Molecular Sequence Data Arabidopsis Transformation Genetic Start codon Gene Expression Regulation Plant Virology Arabidopsis thaliana Phycodnaviridae RNA Messenger Gene Messenger RNA biology Base Sequence Double Stranded DNA Virus biology.organism_classification Plants Genetically Modified Molecular biology Recombinant Proteins Infectious Diseases Paramecium bursaria Enhancer Elements Genetic RNA Plant Nucleic Acid Conformation 5' Untranslated Regions |
| Zdroj: | Virus research. 140(1-2) |
| ISSN: | 0168-1702 |
| Popis: | PBCV-1 (Paramecium bursaria Chlorella virus) is a large double stranded DNA virus that replicates in certain eukaryotic chlorella like green algae. The PBCV-1 A312L gene encodes a 33-kDa protein whose function currently is unknown. The 5′-UTR of the A312L mRNA is 153 nucleotides, longer than the 5′-UTR in any other PBCV-1 gene. The sequence 5′-AAAC was repeated 17 times within 156 bp 5′ to the A312L gene start codon and this sequence was repeated 13 times continuously in the 5′-UTR of the mRNA. Recombinant genes were constructed in vector pBI121 that contained the A312L 5′-UTR, in both the forward and inverse-complement orientations, fused to the GUS gene under the control of the CaMV 35S promoter. These constructs were introduced into Arabidopsis thaliana and the results indicated that the A312L 5′-UTR functions as a translational enhancer only in the forward orientation. Overall, the ratio of GUS enzyme activity to GUS mRNA was 15-fold higher in constructs derived from the A312L 5′-UTR in the forward orientation as compared to constructs containing the 5′-UTR in the inverse-complement orientation or those lacking the A312L 5′-UTR. |
| Databáze: | OpenAIRE |
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