Free radicals upregulate complement expression in rabbit isolated heart
| Autor: | Ruth A. Washington, Elaine J. Tanhehco, Koji Yasojima, Benedict R. Lucchesi, Patrick L. McGeer |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
Xanthine Oxidase Antioxidant Free Radicals Transcription Genetic Physiology medicine.medical_treatment Radical In Vitro Techniques Xanthine chemistry.chemical_compound Downregulation and upregulation Physiology (medical) Gene expression medicine Animals Lagomorpha biology Complement C1q Myocardium Heart Complement C3 Complement System Proteins Complement C9 biology.organism_classification Complement C8 Receptors Complement Complement system Cell biology Gene Expression Regulation chemistry Biochemistry Rabbits Cardiology and Cardiovascular Medicine Complement membrane attack complex |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 279:H195-H201 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.2000.279.1.h195 |
| Popis: | Both free radicals and complement activation can injure tissue. Our study determined whether free radicals alter complement production by the myocardium. Isolated hearts from New Zealand White rabbits were perfused on a Langendorff apparatus and exposed to xanthine (X; 100 μM) plus xanthine oxidase (XO; 8 mU/ml) (X/XO). The free radical-generating system significantly ( P < 0.05) increased C1q and also increased C1r, C3, C8, and C9 transcription compared with controls. Immunohistological examination revealed augmented membrane attack complex deposition on X/XO-treated tissue. X/XO-treated hearts also exhibited significant ( P < 0.05) increases in coronary perfusion pressure and left ventricular end-diastolic pressure and a decrease in left-ventricular developed pressure. N-(2-mercaptopropionyl)-glycine (3 mM), in conjunction with the superoxide dismutase mimetic SC-52608 (100 μM), significantly ( P < 0.05) reduced the upregulation of C1q, C1r, C3, C8, and C9 mRNA expression elicited by X/XO. The antioxidants also ameliorated the deterioration in function caused by X/XO. Local complement activation may represent a mechanism by which free radicals mediate tissue injury. |
| Databáze: | OpenAIRE |
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