Natural variation in stochastic photoreceptor specification and color preference in Drosophila
| Autor: | Elijah Roberts, Caitlin Anderson, Annie Cho, Cyrus Zhou, Daniel Vasiliauskas, Peter DeFord, India Reiss, Haziq Siddiqi, Robert J. Johnston, Alan O. Bergland, Benjamin Mormann, James W. Taylor, Cameron M. Avelis |
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| Přispěvatelé: | Department of Biology, Johns Hopkins University, New york University, Department of Biology, New York University [New York] (NYU), NYU System (NYU)-NYU System (NYU), Department of Biophysics, Johns Hopkins University, Department of Biology, University of Virginia, Institut des Neurosciences Paris-Saclay (NeuroPSI), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
genetic structures [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Regulatory Sequences Nucleic Acid Drosophila Proteins natural variation Biology (General) genes Zinc finger Genetics Regulation of gene expression Behavior Animal D. melanogaster [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior General Neuroscience [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences General Medicine Chromosomes and Gene Expression stochastic cell fate specification Klumpfuss DNA-Binding Proteins Visual Perception Medicine Drosophila Drosophila Protein Research Article Protein Binding chromosomes QH301-705.5 Science Color Biology DNA-binding protein General Biochemistry Genetics and Molecular Biology 03 medical and health sciences developmental biology stem cells Animals Photoreceptor Cells Binding site Transcription factor Gene General Immunology and Microbiology photoreceptor color preference Mutagenesis Insertional 030104 developmental biology Developmental Biology and Stem Cells Gene Expression Regulation Receptors Aryl Hydrocarbon Developmental biology Transcription Factors Spineless |
| Zdroj: | eLife eLife, eLife Sciences Publication, 2017, 6, ⟨10.7554/eLife.29593⟩ eLife, Vol 6 (2017) |
| ISSN: | 2050-084X |
| DOI: | 10.7554/eLife.29593⟩ |
| Popis: | International audience; Each individual perceives the world in a unique way, but little is known about the genetic basis of variation in sensory perception. In the fly eye, the random mosaic of color-detecting R7 photoreceptor subtypes is determined by stochastic ON/OFF expression of the transcription factor Spineless (Ss). In a genome-wide association study, we identified a naturally occurring insertion in a regulatory DNA element in ss that lowers the ratio of SsON to SsOFF cells. This change in photoreceptor fates shifts the innate color preference of flies from green to blue. The genetic variant increases the binding affinity for Klumpfuss (Klu), a zinc finger transcriptional repressor that regulates ss expression. Klu is expressed at intermediate levels to determine the normal ratio of SsON to SsOFF cells. Thus, binding site affinity and transcription factor levels are finely tuned to regulate stochastic expression, setting the ratio of alternative fates and ultimately determining color preference. |
| Databáze: | OpenAIRE |
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