rasoncogene-induced transformation of a rat seminal vesicle epithelial cell line produces a marked increase of adenylate cyclase and protein kinase C activities
| Autor: | A. Furgi, Emilio Chiosi, Magda Marchese, F. Valente, Annamaria Spina, Silvio Naviglio, G. Illiano, Salvatore Metafora |
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| Přispěvatelé: | Spina, Annamaria, Chiosi, Emilio, Naviglio, Silvio, Valente, F, Marchese, M, Furgi, A, Metafora, S, Illiano, G. |
| Rok vydání: | 1993 |
| Předmět: |
Male
G-protein G protein Biophysics ras oncogene Adenylate kinase Biology Biochemistry Cyclase Epithelium GTP-Binding Proteins Protein kinase C Structural Biology Genetics Animals Adenylate cyclase Virulence Factors Bordetella Receptor Molecular Biology Adenosine Diphosphate Ribose Oncogene Effector Colforsin Seminal Vesicles Epithelial Cells Cell Biology Cell Transformation Viral Rats Cell biology Enzyme Activation Genes ras Cell transformation Adenylate Cyclase Toxin Cyclase activity Adenylyl Cyclases Signal Transduction |
| Zdroj: | FEBS Letters. 331:150-154 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(93)80315-l |
| Popis: | Cells transformed by Kirsten murine sarcoma virus (Ki-MSV) have basal adenylate cyclase activity (AC) higher than control cells and comparable level of forskolin-stimulated AC activity. Moreover, a higher protein kinase C (PKC) activity was found to be present in the transformed cells. The molecular mechanism underlying the increase of AC activity was investigated. Our findings strongly suggest that this biochemical event is due to a marked decrease of the α i negative control of the enzyme, even though the α i of transformed cells appears to possess fully functional domains interacting with both the effector enzyme and the agonist-activated receptor. |
| Databáze: | OpenAIRE |
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