Functionalized carbon nanotubes in the brain: cellular internalization and neuroinflammatory responses
| Autor: | Valentin, Ceña, Giuseppe, Bardi, Antonio, Nunes, Lisa, Gherardini, Katie, Bates, Al Jamal, Khuloud T., Claire, Gaillard, Prato, Maurizio, Alberto, Bianco, Tommaso, Pizzorusso, Kostas, Kostarelos |
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| Přispěvatelé: | Bardi G, G, Nunes, A, Gherardini, L, Bates, K, Al-Jamal, Kt, Gaillard, C, Prato, M, Bianco, A, Pizzorusso, T, Kostarelos, K, Immunopathologie et chimie thérapeutique (ICT), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Valentin, Ceña, Giuseppe, Bardi, Antonio, Nune, Lisa, Gherardini, Katie, Bate, Khuloud T., Al Jamal, Claire, Gaillard, Prato, Maurizio, Alberto, Bianco, Tommaso, Pizzorusso, Kostas, Kostarelos |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Gene Expression
lcsh:Medicine [CHIM.THER]Chemical Sciences/Medicinal Chemistry 02 engineering and technology Settore BIO/09 - Fisiologia Stereotaxic Techniques Mice chemically induced metabolism pathology Internalization lcsh:Science media_common Neurons 0303 health sciences Multidisciplinary CD11b Antigen Glial fibrillary acidic protein biology Microglia Chemistry Brain chemistry toxicity 021001 nanoscience & nanotechnology medicine.anatomical_structure Cytokines Carbon Nanotubes Female 0210 nano-technology Cell activation Oxidation-Reduction Research Article media_common.quotation_subject Central nervous system Nerve Tissue Proteins Gene delivery drug effects Proinflammatory cytokine 03 medical and health sciences Neuroinflamation Glial Fibrillary Acidic Protein medicine Animals 030304 developmental biology Injections Intraventricular Inflammation Nanotubes Carbon neuroflammation drug delivery lcsh:R Biological Transport genetics Mice Inbred C57BL Astrocytes Stereotaxic technique Immunology biology.protein Biophysics lcsh:Q Chimie/Chimie thérapeutique Biomarkers |
| Zdroj: | PLoS ONE, Vol 8, Iss 11, p e80964 (2013) PLoS ONE PLoS ONE, Public Library of Science, 2013, 8 (11), pp.e80964. ⟨10.1371/journal.pone.0080964⟩ |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0080964⟩ |
| Popis: | The potential use of functionalized carbon nanotubes (f-CNTs) for drug and gene delivery to the central nervous system (CNS) and as neural substrates makes the understanding of their in vivo interactions with the neural tissue essential. The aim of this study was to investigate the interactions between chemically functionalized multi-walled carbon nanotubes (f-MWNTs) and the neural tissue following cortical stereotactic administration. Two different f-MWNT constructs were used in these studies: shortened (by oxidation) amino-functionalized MWNT (oxMWNT-NH3(+)) and amino-functionalized MWNT (MWNT-NH3(+)). Parenchymal distribution of the stereotactically injected f-MWNTs was assessed by histological examination. Both f-MWNT were uptaken by different types of neural tissue cells (microglia, astrocytes and neurons), however different patterns of cellular internalization were observed between the nanotubes. Furthermore, immunohistochemical staining for specific markers of glial cell activation (GFAP and CD11b) was performed and secretion of inflammatory cytokines was investigated using real-time PCR (qRT-PCR). Injections of both f-MWNT constructs led to a local and transient induction of inflammatory cytokines at early time points. Oxidation of nanotubes seemed to induce significant levels of GFAP and CD11b over-expression in areas peripheral to the f-MWNT injection site. These results highlight the importance of nanotube functionalization on their interaction with brain tissue that is deemed critical for the development nanotube-based vector systems for CNS applications. The potential use of functionalized carbon nanotubes (f-CNTs) for drug and gene delivery to the central nervous system (CNS) and as neural substrates makes the understanding of their in vivo interactions with the neural tissue essential. The aim of this study was to investigate the interactions between chemically functionalized multi-walled carbon nanotubes (f-MWNTs) and the neural tissue following cortical stereotactic administration. Two different f-MWNT constructs were used in these studies: shortened (by oxidation) amino-functionalized MWNT (oxMWNT-NH3+) and amino-functionalized MWNT (MWNT-NH3+). Parenchymal distribution of the stereotactically injected f-MWNTs was assessed by histological examination. Both f-MWNT were uptaken by different types of neural tissue cells (microglia, astrocytes and neurons), however different patterns of cellular internalization were observed between the nanotubes. Furthermore, immunohistochemical staining for specific markers of glial cell activation (GFAP and CD11b) was performed and secretion of inflammatory cytokines was investigated using real-time PCR (qRT-PCR). Injections of both f-MWNT constructs led to a local and transient induction of inflammatory cytokines at early time points. Oxidation of nanotubes seemed to induce significant levels of GFAP and CD11b over-expression in areas peripheral to the f-MWNT injection site. These results highlight the importance of nanotube functionalization on their interaction with brain tissue that is deemed critical for the development nanotube-based vector systems for CNS applications. © 2013 Bardi et al. |
| Databáze: | OpenAIRE |
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