A multiplex assay for detection of SHIV plasma and mucosal IgG and IgA

Autor: Tyana Singletary, Krystin Ambrose Price, James M. Smith, Ivana Parker, Kelly A. Curtis, Priya Srinivasan
Rok vydání: 2017
Předmět:
0301 basic medicine
Time Factors
Anti-HIV Agents
Phosphorous Acids
030106 microbiology
Immunology
Simian Acquired Immunodeficiency Syndrome
Simian
HIV Antibodies
medicine.disease_cause
03 medical and health sciences
Immune system
Predictive Value of Tests
medicine
Immunology and Allergy
Animals
Avidity
Multiplex
Serologic Tests
Immunity
Mucosal
Immunoassay
Mucous Membrane
biology
medicine.diagnostic_test
Adenine
Mucous membrane
Reproducibility of Results
Simian immunodeficiency virus
Viral Load
biology.organism_classification
Virology
Immunity
Humoral
Immunoglobulin A
Administration
Intravaginal
Disease Models
Animal
medicine.anatomical_structure
Seroconversion
Immunoglobulin G
Host-Pathogen Interactions
Vagina
biology.protein
Adenoviruses
Simian
Female
Antibody
Macaca nemestrina
Biomarkers
Zdroj: Journal of immunological methods. 450
ISSN: 1872-7905
Popis: Evaluating antibody maturation provides valuable data to characterize immune responses to HIV infection and can provide insight into biomedical intervention efficacy. It is important to develop assays that evaluate antibody maturation in both plasma and mucosal compartments. The nonhuman primate model provides a controlled system to collect temporal data that are integral to assessing intervention strategies. We report the development of a novel multiplex assay, based on the Bio-Plex platform, to evaluate plasma and mucosal IgG and IgA avidity and maturation against simian/human immunodeficiency virus (SHIV) in this controlled system. Vaginal mucosa and plasma samples were collected from a prior study evaluating the efficacy of a tenofovir disoproxil fumarate (TDF) intravaginal ring (IVR) against SHIVSF162P3 challenge in female pigtailed macaques. For validation of the multiplex assay, specimens from six SHIV-infected placebo animals and one TDF breakthrough animal were evaluated. For SHIV and HIV envelope analytes, antibody levels and avidity in both compartments continued to mature post-infection. Maturation of IgG and IgA levels was similar in each compartment, however, mucosal antibody levels tended to be more variable. This SHIV assay elucidates IgG/IgA antibody kinetics in the plasma and vaginal mucosa and will be a valuable tool in vaccine and other biomedical intervention studies in the nonhuman primate model.
Databáze: OpenAIRE
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