Effects of serum-isolated vs synthetic bilirubin-albumin complexes on dye-binding methods for estimating serum albumin
| Autor: | Hiroshi Ihara, Yutaka Aoki, Hiroomi Nakamura, Mitsutaka Yoshida, Tsugutoshi Aoki |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Adult
biology Chemistry Bilirubin Dye binding Biochemistry (medical) Clinical Biochemistry Serum albumin Albumin Serum Albumin Human medicine.disease Colorimetry (chemical method) chemistry.chemical_compound Bromcresol Purple Biochemistry biology.protein Bromcresol Green medicine Humans Hypoalbuminemia Coloring Agents Chromatography High Pressure Liquid Serum Albumin |
| Zdroj: | Clinical Chemistry. 37:1269-1272 |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | Bush and Reed reported (Clin Chem 1987;33:821-3) that the reaction of albumin with bromcresol purple but not with bromcresol green underestimated the concentration of albumin in synthetically obtained bilirubin-albumin (Bd) by 29%. Their unproven assumption was that chemically synthesized Bd behaved in a manner indistinguishable from the natural Bd in icteric serum. Here we verify that Bd, whether synthetically obtained or isolated from serum, causes an underestimation of albumin in the bromcresol purple but not in the bromcresol green method. The molar ratio of Bd from either source to underestimated albumin approximates 1.0, suggesting that one molecule of Bd would react equivalently to a molecule of albumin in the bromcresol purple method. This underestimation might falsely suggest hypoalbuminemia in patients with increased serum Bd. |
| Databáze: | OpenAIRE |
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