Real-Time Observation of Multiple-Protein Complex Formation with Single-Molecule FRET

Autor:	Changbong Hyeon, Tae-Young Yoon, Yeon-Kyun Shin, Woori Bae, Mal-Gi Choi
Rok vydání:	2013
Předmět:	Chemistry Extramural Kinetics General Chemistry Plasma protein binding Single-molecule FRET Biochemistry Article Catalysis Crystallography Colloid and Surface Chemistry Förster resonance energy transfer Biophysics Protein complex formation Attachment protein SNARE complex
Zdroj:	Journal of the American Chemical Society. 135:10254-10257
ISSN:	1520-5126 0002-7863
DOI:	10.1021/ja404276g
Popis:	Current single-molecule techniques do not permit the real-time observation of multiple proteins interacting closely with each other. We here report an approach enabling us to determine the single-molecule fluorescence resonance energy transfer (FRET) kinetics of multiple protein–protein interactions occurring far below the diffraction limit. We observe a strongly cooperative formation of multimeric soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes, which suggests that formation of the first SNARE complex triggers a cascade of SNARE complex formation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d166ee6655c310355d88a1f157cb82f https://doi.org/10.1021/ja404276g Zobrazit plný text záznamu