The 5′ Flanking Region of the Human Bone Morphogenetic Protein-7 Gene
| Autor: | Haimanti Dorai, Kuber T. Sampath, Alyssa A. Shepard, Hermann Oppermann, Simon S. Jones, Engin Ozkaynak, Keming Lin |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Therapeutic gene modulation
Bone Morphogenetic Protein 7 Molecular Sequence Data 5' flanking region Biophysics Gene Expression Biology Transfection Biochemistry Bone and Bones Dinoprostone Cell Line Species Specificity Genes Reporter Transforming Growth Factor beta Sequence Homology Nucleic Acid Gene expression Animals Humans Amino Acid Sequence Gene Silencing RNA Messenger Cloning Molecular Luciferases Promoter Regions Genetic Molecular Biology DNA Primers Reporter gene Base Sequence Bone morphogenetic protein 10 Cell Biology Molecular biology Rats BMPR1B Bone morphogenetic protein 7 Bone morphogenetic protein 6 Parathyroid Hormone Bone Morphogenetic Proteins |
| Zdroj: | Biochemical and Biophysical Research Communications. 282:823-831 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.2001.4645 |
| Popis: | We describe here the cloning and characterization of the 5' flanking region of the human Bone Morphogenetic Protein-7 (BMP-7) gene from a 3.3 kb genomic DNA fragment. Functional analysis by transient transfection using the luciferase reporter gene indicated that this region had a low basal promoter activity in human Wilm's tumor derived renal (G401) and rat osteoblast (ROS 17/2.8) cell lines. Sequential deletion analysis of the promoter revealed sequences whose presence correlated with decreased expression of the reporter gene. Coexpression of transcription factors involved in epithelial/mesenchymal interactions during kidney and eye development dramatically stimulated the expression of the reporter gene from the putative BMP-7 promoter. Finally, a subset of agents that upregulated the expression of the reporter gene from the cloned promoter were also shown to increase the expression of the endogenous BMP-7 in G401 and ROS cell lines in vitro. |
| Databáze: | OpenAIRE |
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