Are methicillin-resistant Staphylococcus aureus that produce Panton-Valentine leucocidin (PVL) found among residents of care homes?

Autor: Gillian Hodgson, Ben Barr, Carolyne S. Smith, D. S. Tompkins, Mark H. Wilcox, Bob Darby, P. Parnell
Rok vydání: 2008
Předmět:
DNA
Bacterial
Microbiology (medical)
Staphylococcus aureus
medicine.medical_specialty
Micrococcaceae
Genotype
Bacterial Toxins
Exotoxins
Microbial Sensitivity Tests
medicine.disease_cause
Staphylococcal infections
Polymerase Chain Reaction
Microbiology
Leukocidins
Epidemiology
Pulsed-field gel electrophoresis
Cluster Analysis
Humans
Medicine
Pharmacology (medical)
Pharmacology
Cross Infection
biology
business.industry
Staphylococcal Infections
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
biology.organism_classification
medicine.disease
DNA Fingerprinting
Methicillin-resistant Staphylococcus aureus
United Kingdom
Bacterial Typing Techniques
Electrophoresis
Gel
Pulsed-Field
Nursing Homes
Nasal Mucosa
Infectious Diseases
Cohort
Methicillin Resistance
Panton–Valentine leukocidin
business
Zdroj: Journal of Antimicrobial Chemotherapy. 62:968-972
ISSN: 1460-2091
0305-7453
DOI: 10.1093/jac/dkn326
Popis: Objectives: Panton–Valentine leucocidin (PVL)-positive Staphylococcus aureus are responsible for causing skin and soft tissue infections, with the potential to cause severe invasive disease. Recently, methicillin-resistant Staphylococcus aureus (MRSA) strains that produce PVL have emerged in the community. As residents of care homes are a key group at risk of MRSA colonization and infection, we have examined the epidemiology of MRSA in three large cohorts of residents in urban care homes to establish whether PVL-positive MRSA strains are present in this setting. Methods: Nasal swabs (n 5 3037) collected from consenting residents of 69 care homes in Leeds, UK, were screened for MRSA using chromogenic agar over three periods (June–August 2005, November– December 2006 and October–November 2007). PCR amplification was used to detect genes encoding PVL. Antibiogram profile and PFGE were also used to characterize MRSA isolates (n 5 601). Results: MRSA prevalence was 21%, 20% and 19% in each cohort, respectively. The majority of the isolates were related epidemiologically to the predominant local nosocomial epidemic MRSA strain, EMRSA-15 (78%). No isolate carried the genes encoding PVL. Twelve percent of the isolates (n 5 74) had increased susceptibility to non-b-lactam agents and were distributed across 31 care homes. Conclusions: MRSA strains that produced PVL were not found to be colonizing residents of care homes between 2005 and 2007. Continued surveillance is, however, necessary to understand the interaction between MRSA in care homes and hospitals, especially to reduce the chance that the former may amplify community-associated MRSA strains.
Databáze: OpenAIRE
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