Imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells
| Autor: | Takahiro Ochiya, Takao Yasui, Kaoru Suzuki, Tetsuya Ishikawa, Yoshinobu Baba, Tomoko Arimoto, Hiroshi Yukawa, Noritada Kaji, Keita Aoki |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Vascular Endothelial Growth Factor A
0301 basic medicine Carcinoma Hepatocellular Angiogenesis Science Exosomes Article Umbilical vein Neovascularization 03 medical and health sciences chemistry.chemical_compound Immune system Heat shock protein Human Umbilical Vein Endothelial Cells medicine Humans HSP70 Heat-Shock Proteins neoplasms Multidisciplinary Neovascularization Pathologic Chemistry Liver Neoplasms Hep G2 Cells NKG2D digestive system diseases Microvesicles Gene Expression Regulation Neoplastic Vascular endothelial growth factor MicroRNAs 030104 developmental biology NK Cell Lectin-Like Receptor Subfamily K Cancer research Medicine medicine.symptom |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-24563-0 |
| Popis: | Hepatocellular carcinoma (HCC) is a typical hyper-vascular tumor, so the understanding the mechanisms of angiogenesis in HCC is very important for its treatment. However, the influence of the exosomes secreted from HCC cells (HCC-exosomes) on angiogenesis remains poorly understood. We herein examined the effects of the exosomes secreted from HepG2 cells (HepG2-exosomes) on the lumen formation of human umbilical vein endothelial cells (HUVECs) by the imaging of angiogenesis. The degree of lumen formation of HUVECs was dependent on the number of HepG2-exosomes. The HepG2-exosomes expressed NKG2D, an activating receptor for immune cells, and HSP70, a stress-induced heat shock protein associated with angiogenesis through the vascular endothelial growth factor (VEGF) receptor. In addition, the HepG2-exosomes contained several microRNAs (miRNAs) reported to exist in the serum of HCC patients. These results suggest that the HCC-exosomes play an important role in angiogenesis. Further studies on the function of HCC-exosomes may provide a new target for HCC treatment. |
| Databáze: | OpenAIRE |
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