Inactivation of lipid-enveloped viruses in proteins by caprylate
| Autor: | R. L. Seng, J. L. Lundblad |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Sindbis virus
viruses Size-exclusion chromatography medicine.disease_cause Antiviral Agents Virus chemistry.chemical_compound Viral envelope medicine Humans Biological Products biology Proteins Biological activity Hematology General Medicine Hydrogen-Ion Concentration biology.organism_classification Lipids Herpes simplex virus chemistry Biochemistry Vesicular stomatitis virus Vaccinia Caprylates Drug Contamination |
| Zdroj: | Vox sanguinis. 60(2) |
| ISSN: | 0042-9007 |
| Popis: | The use of caprylate for the inactivation of lipid-enveloped viruses in biologically active proteins both plasma derived and produced by cell culture was evaluated. Viruses consisted of herpes simplex virus type I, vesicular stomatitis virus, vaccinia virus, and Sindbis virus. Utilizing the dissociation reaction and varying the concentration of the ionized form of caprylate, a specific amount of the nonionized form of caprylate was maintained over a wide pH range. Virus-spiked protein solutions contacted with caprylate provide rapid virus inactivation under a variety of conditions while maintaining the integrity of the respective protein or activity. With the exception of coagulation factor AHF, protein and biological activity yield were essentially quantitative. Caprylate is removed after treatment by size exclusion chromatography or anion/cation exchange adsorption of the protein, followed by buffer wash. |
| Databáze: | OpenAIRE |
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