Hypoxia/reoxygenation stimulates endothelium to promote neutrophil adhesion
| Autor: | Claude Bonne, O. Palluy, Lucette Morliere, G. Modat, Jean-Christophe Gris |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Adult
P-selectin Endothelium Free Radicals Neutrophils Platelet Membrane Glycoproteins In Vitro Techniques medicine.disease_cause Biochemistry Physiology (medical) E-selectin medicine Cell Adhesion Humans Cell adhesion Cells Cultured biology Cell adhesion molecule Chemistry Free Radical Scavengers Cell Hypoxia Cell biology Endothelial stem cell Oxygen P-Selectin medicine.anatomical_structure Myeloperoxidase biology.protein Endothelium Vascular E-Selectin Cell Adhesion Molecules Oxidative stress |
| Zdroj: | Free radical biologymedicine. 13(1) |
| ISSN: | 0891-5849 |
| Popis: | An in vitro model was designed to study the role of ischemia/reperfusion and endothelium-derived oxygen free radicals on neutrophil adhesion, with particular interest in the endothelial adhesion molecules involved. Human umbilical vein endothelial cells were submitted to 5 h hypoxia followed by various times (20 min to 24 h) of reoxygenation. Human resting neutrophils were added to monolayers for the last 15 min of reoxygenation. Adherence was evaluated by myeloperoxidase assay. Under these conditions, we found an increased adhesion of neutrophils with two peaks after 20 min and 4 h reoxygenation. This was correlated with the respective expression of the preformed granule membrane protein 140 (GMP-140) and of the de novo synthesized endothelial leukocyte adhesion molecule 1 (ELAM-1) on endothelial surface. Superoxide dismutase and/or catalase, or oxypurinol added to cultures before hypoxia efficiently prevented neutrophil adhesion. These results underline the crucial role played by endothelial oxy radicals at reoxygenation in adhesion of leukocytes, which could lead to an amplification of the oxidative stress injury. The protection offered by free radical scavengers emphasizes the potential therapeutic use of antioxidants in postischemic vascular disorders. |
| Databáze: | OpenAIRE |
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