Characterization of monoclonal antibodies specific for rabbit renal brush-border hydrolases: application to immunohistological localization
| Autor: | Michel Tauc, Philippe Poujeol, Pierre Ronco, Alain Vandewalle, F. Chatelet, Pierre J. Verroust |
|---|---|
| Přispěvatelé: | Physiologie cellulaire et moléculaire des systèmes intégrés (PCMSI), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Direction Innovation, Recherche et Développement, Chambre Régionale d'Agriculture des Pays de la Loire, Service de Néphrologie et Dialyses [CHU Tenon], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
MESH: Microvilli
Hydrolases [SDV]Life Sciences [q-bio] MESH: Rabbits Kidney Aminopeptidases Chromatography Affinity MESH: Antibodies Monoclonal MESH: Animals MESH: Endopeptidases Neprilysin 0303 health sciences biology Microvilli MESH: Neprilysin 030302 biochemistry & molecular biology Antibodies Monoclonal MESH: Chromatography Affinity Immunohistochemistry 3. Good health MESH: Hydrolases medicine.anatomical_structure Biochemistry Female Rabbits Anatomy Antibody MESH: Dipeptidyl-Peptidases and Tripeptidyl-Peptidases Histology Brush border medicine.drug_class Dipeptidyl Peptidase 4 Immunocytochemistry MESH: Microscopy Electron CD13 Antigens Peptidyl-Dipeptidase A Monoclonal antibody MESH: Aminopeptidases 03 medical and health sciences Affinity chromatography Endopeptidases medicine Animals Dipeptidyl-Peptidases and Tripeptidyl-Peptidases 030304 developmental biology MESH: CD13 Antigens MESH: Immunohistochemistry MESH: Kidney MESH: Dipeptidyl Peptidase 4 Microscopy Electron MESH: Peptidyl-Dipeptidase A biology.protein MESH: Female |
| Zdroj: | Journal of Histochemistry and Cytochemistry Journal of Histochemistry and Cytochemistry, Histochemical Society, 2017, 36 (5), pp.523-532. ⟨10.1177/36.5.2895788⟩ |
| ISSN: | 0022-1554 |
| DOI: | 10.1177/36.5.2895788⟩ |
| Popis: | International audience; By use of immunodepletion studies, we characterized four monoclonal antibodies reactive with rabbit brush-border (BB) as specific for aminopeptidase N (AP), dipeptidylpeptidase IV (DPPIV), neutral endopeptidase (EP), and angiotensin-converting enzyme (ACE), and we used these antibodies for immunohistochemical detection of these four hydrolases. Expression within the kidney was studied by light and electron microscopy. All four hydrolases are expressed on the various segments of the proximal tubule. In addition, EP and DPPIV are detectable on visceral epithelial cells of the glomerulus and AP on the cells of Bowman's capsule. Outside the kidney, the four hydrolases are expressed within the digestive and genital tracts, where AP, EP, and DPPIV predominate on epithelial structures, whereas ACE is essentially located in vascular structures. The latter localization is also characteristic of ACE in the other organs studied, where clear-cut systematic distribution of the other hydrolases was often difficult to demonstrate. In addition, AP, DPPIV, and EP were detected on lymphoid cells. As compared to reports of data obtained essentially by enzymatic or immunoradiometric assays, these observations suggest considerable interspecies variations of extrarenal expression of the major BB hydrolases. This should be taken into account in attempting to define a general physiological role for a given enzyme. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|