An Experimentally Derived Database of Candidate Ras-Interacting Proteins
| Autor: | Ptak C, Mark H. Ginsberg, Donald F. Hunt, Erin D. Jeffery, J. Shabanowitz, Lawrence E. Goldfinger, Jaewon Han, Haling, Fox Jw, Nicholas E. Sherman |
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| Rok vydání: | 2007 |
| Předmět: |
Cell signaling
Recombinant Fusion Proteins CHO Cells GTPase Plasma protein binding Biology Tandem mass spectrometry computer.software_genre Biochemistry DNA-binding protein Chromatography Affinity Article Mice Cricetulus Cricetinae Animals Databases Protein Cytoskeleton Telomere-binding protein Database Chinese hamster ovary cell rap1 GTP-Binding Proteins General Chemistry Cell biology NIH 3T3 Cells ras Proteins computer Protein Binding |
| Zdroj: | Journal of Proteome Research. 6:1806-1811 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr060630l |
| Popis: | We used a TAP-tag approach to identify candidate binding proteins for the related Ras family GTPases: H-Ras, R-Ras, and Rap1A. Protein complexes were isolated from mouse fibroblasts, and component proteins were identified by a combination of nanoflow HPLC and tandem mass spectrometry. H-Ras was found to associate with numerous cytoskeletal proteins including talin-1. R-Ras and Rap1A each associated with various signaling molecules, many of which are membrane-associated. Thus, we have established the first database of potential Ras interactors in mammalian cells. |
| Databáze: | OpenAIRE |
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