Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
| Autor: | Ben Zhong Tang, Sheng Xie, Alex Y. H. Wong, Sijie Chen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Gel electrophoresis
Silver Staining Chromatography General Immunology and Microbiology Silver Staining Method General Chemical Engineering General Neuroscience Polyacrylamide Acrylic Resins Proteins Reproducibility of Results Fluorescence Stain General Biochemistry Genetics and Molecular Biology Staining Silver stain Electrophoresis chemistry.chemical_compound chemistry Electrophoresis Polyacrylamide Gel Fluorescent Dyes |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/58669 |
| Popis: | Silver staining is a colorimetric technique widely used to visualize protein bands in polyacrylamide gels following sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The classic silver stains have certain drawbacks, such as high background staining, poor protein recovery, low reproducibility, a narrow linear dynamic range for quantification, and limited compatibility with mass spectrometry (MS). Now, with the use of a fluorogenic Ag+ probe, TPE-4TA, we developed a fluorescent silver staining method for the total protein visualization in polyacrylamide gels. This new stain avoids the troublesome silver reduction step in traditional silver stains. Moreover, the fluorescent silver stain demonstrates good reproducibility, sensitivity, and linear quantification in protein detection, making it a useful and practical protein gel stain. |
| Databáze: | OpenAIRE |
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