Stabilizing factor(s) of nitric oxide (NO) synthetase
| Autor: | Kunihiko Kosuga, Kazuaki Hiki, Yoshiki Yui, Ryuichi Hattori, Hirosi Eizawa, Chuichi Kawai |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Hot Temperature
Nitric oxide synthetase Neutrophils Neutrophile Biophysics Granulocyte Biology Biochemistry Nitric oxide chemistry.chemical_compound Cytosol Peritoneum Enzyme Stability Polymorphonuclear Neutrophils medicine Animals Molecular Biology chemistry.chemical_classification Cell Biology Rats Kinetics Enzyme medicine.anatomical_structure chemistry Amino Acid Oxidoreductases Nitric Oxide Synthase |
| Zdroj: | Biochemical and Biophysical Research Communications. 172:705-708 |
| ISSN: | 0006-291X |
| Popis: | We found that the cytosol of rat peritoneal polymorphonuclear neutrophils contains factor(s) that can stabilize an unstable enzyme, nitric oxide synthetase, in the cytosol. This enzyme has been purified to a single protein from the cytosol. Its half-life was 3 hours at 4 degrees C and was prolonged to greater than 24 hours by the stabilizing factor in the cytosol. The molecular weight of the stabilizing factor was greater than 100,000. Its activity was lost by the treatment with heating or alkali for 1 min or with acid for 5 min. It did not adhere to the carboxymethyl or diethylaminoethyl column at neutral pH. This stabilizing factor(s) may play a role in the regulation of the nitric oxide synthetase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|