Generation of Fibrodysplasia ossificans progressiva and control integration free iPSC lines from periodontal ligament fibroblasts
| Autor: | Jan Coen Netelenbos, Elisabeth M.W. Eekhoff, T. J. de Vries, Christian Freund, Karoly Szuhai, Danielle de Jong, Gonzalo Sanchez-Duffhues, Harald Mikkers, Marie-José Goumans, P. ten Dijke, R. van Es, Nathalie Bravenboer |
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| Přispěvatelé: | Internal medicine, ACS - Diabetes & metabolism, AGEM - Endocrinology, metabolism and nutrition, Amsterdam Movement Sciences - Rehabilitation & Development, Parodontologie (OII, ACTA), Periodontology |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Periodontal Ligament Induced Pluripotent Stem Cells Cell Culture Techniques Bone morphogenetic protein Cell Line 03 medical and health sciences Young Adult 0302 clinical medicine SDG 3 - Good Health and Well-being medicine Periodontal fiber Humans Fibroblast Induced pluripotent stem cell lcsh:QH301-705.5 biology Base Sequence Point mutation Reproducibility of Results General Medicine Cell Biology Fibroblasts biology.organism_classification medicine.disease Sendai virus 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Fibrodysplasia ossificans progressiva Cancer research Heterotopic ossification Female 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cell Research, 41. Elsevier Stem Cell Research, 41. ELSEVIER Stem Cell Research, 41:101639. Elsevier Stem Cell Research, Vol 41, Iss, Pp-(2019) Sanchez-Duffhues, G, Mikkers, H, de Jong, D, Szuhai, K, de Vries, T J, Freund, C, Bravenboer, N, van Es, R J J, Netelenbos, J C, Goumans, M J, Eekhoff, E M W & ten Dijke, P 2019, ' Generation of Fibrodysplasia ossificans progressiva and control integration free iPSC lines from periodontal ligament fibroblasts ', Stem Cell Research, vol. 41, 101639 . https://doi.org/10.1016/j.scr.2019.101639 Sanchez-Duffhues, G, Mikkers, H, de Jong, D, Szuhai, K, de Vries, T J, Freund, C, Bravenboer, N, van Es, R J J, Netelenbos, J C, Goumans, M J, Eekhoff, E M W & ten Dijke, P 2019, ' Generation of fibrodysplasia ossificans progressiva and control integration free iPSC lines from periodontal ligament fibroblasts ', Stem Cell Research, vol. 41, 101639 . https://doi.org/10.1016/j.scr.2019.101639 |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2019.101639 |
| Popis: | Fibrodysplasia ossificans progressiva (FOP) is a very rare devastating heterotopic ossification disorder, classically caused by a heterozygous single point mutation (c.617G>A) in the ACVR1gene, encoding the Bone morphogenetic protein (BMP) type I receptor, also termed activin receptor-like kinase (ALK)2. FOP patients develop heterotopic ossification episodically in response to inflammatory insults, thereby compromising tissue sampling and the development of in vitro surrogate models for FOP. Here we describe the generation and characterization of a control and a classical FOP induced pluripotent stem cell (iPSC) line derived from periodontal ligament fibroblast cells using Sendai virus vectors. |
| Databáze: | OpenAIRE |
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