Elevated microRNA-7 inhibits proliferation and tumor angiogenesis and promotes apoptosis of gastric cancer cells via repression of Raf-1
| Autor: | Wanting Zeng, E. Li, Zewa Liu, Jing Lin, Shasha Liao, Xiaohua Wu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Expression of Concern 0301 basic medicine Tumor angiogenesis Mice Nude Apoptosis Biology 03 medical and health sciences 0302 clinical medicine Cell Movement Stomach Neoplasms Cell Line Tumor microRNA Animals Humans Neoplasm Invasiveness Molecular Biology Psychological repression Aged Cell Proliferation Mice Inbred BALB C Neovascularization Pathologic Cell Biology Middle Aged MicroRNA 7 Neoadjuvant Therapy Gene Expression Regulation Neoplastic Proto-Oncogene Proteins c-raf MicroRNAs 030104 developmental biology Chemotherapy Adjuvant Case-Control Studies 030220 oncology & carcinogenesis Cancer cell Cancer research Female Microvascular Density Signal Transduction Developmental Biology |
| Zdroj: | Cell Cycle |
| ISSN: | 1551-4005 1538-4101 |
| DOI: | 10.1080/15384101.2020.1807670 |
| Popis: | Since the essential involvement of microRNAs (miRNAs) in the development and progression of GC, the study was for the exploration of the value of microRNA-7 (miR-7) in the evaluation of neoadjuvant chemotherapy for gastric cancer (GC) and its effects on apoptosis, proliferation and angiogenesis of GC.miR-7 expression in serum of GC patients before and after neoadjuvant chemotherapy were detected to explore its role in neoadjuvant chemotherapy of GC. The GC cells were transfected with miR-7 mimics/inhibitors, or siRNA-Raf-1 to figure out their roles in proliferation, migration, invasion, cycle distribution and apoptosis. Tumor xenograft was conducted to test tumor growth. Microvessel density (MVD) in tumors was tested by immunohistochemical staining.miR-7 expression in serum of GC patients was lower than that of healthy controls while it was elevated after neoadjuvant chemotherapy. Moreover, higher miR-7 expression was exhibited in chemotherapy-effective patients rather than chemotherapy-ineffective patients (Our study highlights that elevated miR-27a indicates the good efficacy of neoadjuvant chemotherapy in GC and miR-7 targets Raf-1 to suppress tumor development and angiogenesis of GC cells. |
| Databáze: | OpenAIRE |
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