Silver, titanium dioxide, and zinc oxide nanoparticles trigger miRNA/isomiR expression changes in THP-1 cells that are proportional to their health hazard potential

Autor: Hani El-Nezami, Joseph Ndika, Vittorio Fortino, Umair Seemab, Piia Karisola, Wing-Lam Poon, Harri Alenius
Přispěvatelé: HUMI - Human Microbiome Research, Research Programs Unit, University of Helsinki, Faculty of Medicine, Staff Services
Rok vydání: 2019
Předmět:
MECHANISM
congenital
hereditary
and neonatal diseases and abnormalities
Silver
THP-1 Cells
Biomedical Engineering
Nanoparticle
chemistry.chemical_element
Metal Nanoparticles
macromolecular substances
02 engineering and technology
Zinc
010501 environmental sciences
Toxicology
01 natural sciences
Metal-based nanoparticle
chemistry.chemical_compound
IsomiR
Health hazard
isomiR
microRNA
Humans
TUMOR-SUPPRESSOR
THP1 cell line
RNA
Messenger
Particle Size
miRNA
0105 earth and related environmental sciences
NANOMATERIALS
TOOLS
Titanium
Gene Expression Profiling
technology
industry
and agriculture
PATHWAYS
021001 nanoscience & nanotechnology
humanities
Cell biology
MicroRNAs
chemistry
Gene Expression Regulation
bio-nano reactivity
Titanium dioxide
1182 Biochemistry
cell and molecular biology
RISK-ASSESSMENT
3111 Biomedicine
MIR-142-3P FUNCTIONS
Zinc Oxide
0210 nano-technology
Zdroj: Nanotoxicology. 13(10)
ISSN: 1743-5404
Popis: After over a decade of nanosafety research, it is indisputable that the vast majority of nano-sized particles induce a plethora of adverse cellular responses - the severity of which is linked to the material's physicochemical properties. Differentiated THP-1 cells were previously exposed for 6 h and 24 h to silver, titanium dioxide, and zinc oxide nanoparticles at the maximum molar concentration at which no more than 15% cellular cytotoxicity was observed. All three nanoparticles differed in extent of induction of biological pathways corresponding to immune response signaling and metal ion homeostasis. In this study, we integrated gene and miRNA expression profiles from the same cells to propose miRNA biomarkers of adverse exposure to metal-based nanoparticles. We employed RNA sequencing together with a quantitative strategy that also enables analysis of the overlooked repertoire of length and sequence miRNA variants called isomiRs. Whilst only modest changes in expression were observed within the first 6 h of exposure, the miRNA/isomiR (miR) profiles of each nanoparticle were unique. Via canonical correlation and pathway enrichment analyses, we identified a co-regulated miR-mRNA cluster, predicted to be highly relevant for cellular response to metal ion homeostasis. These miRs were annotated to be canonical or variant isoforms of hsa-miR-142-5p, -342-3p, -5100, -6087, -6894-3p, and -7704. Hsa-miR-5100 was differentially expressed in response to each nanoparticle in both the 6 h and 24 h exposures. Taken together, this co-regulated miR-mRNA cluster could represent potential biomarkers of sub-toxic metal-based nanoparticle exposure.
Databáze: OpenAIRE
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