Alterations of cytological and karyological profile of human mesenchymal stem cells during in vitro culturing
| Autor: | P. V. Kruglyakov, Kuznetsova Tv, U. A. Taminkina, A. V. Voskresenskaya, D. G. Polyntsev, Olga A. Efimova, A. A. Pendina, A. S. Grigorian |
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| Rok vydání: | 2010 |
| Předmět: |
Chromosome Aberrations
Pathology medicine.medical_specialty Mesenchymal stem cell Cell Culture Techniques Clinical uses of mesenchymal stem cells Amniotic stem cells Mesenchymal Stem Cells General Medicine Biology General Biochemistry Genetics and Molecular Biology Immunophenotyping medicine.anatomical_structure Cancer stem cell Immunology medicine Humans Bone marrow Stem cell Cells Cultured Stem cell transplantation for articular cartilage repair Adult stem cell |
| Zdroj: | Bulletin of experimental biology and medicine. 150(1) |
| ISSN: | 1573-8221 |
| Popis: | Transplantation of human bone marrow mesenchymal stem cells is considered as a promising therapeutic approach to the therapy of many diseases. However, the problem of possible alterations of the properties of mesenchymal stem cells during their expansion in in vitro cultures before transplantation is not solved. In our study, one of two hundred examined cultures of mesenchymal stem cell cultures derived from donors without bone marrow pathologies and developed under standard culturing conditions demonstrated spontaneous disturbances in morphology, proliferation, and karyotype at early passages. The cells of this abnormal culture retained immunophenotype characteristic of normal mesenchymal stem cells, but some of them (15-25%) had numerous numerical and structural chromosome aberrations. |
| Databáze: | OpenAIRE |
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