Cav2-Type Calcium Channels Encoded bycacRegulate AP-Independent Neurotransmitter Release at Cholinergic Synapses in AdultDrosophilaBrain
| Autor: | Xicui Sun, Huaiyu Gu, Jorge Iniguez, Jorge M. Campusano, Hailing Su, Andy An Hoang, Shaojuan Amy Jiang, Monica Lavian, Diane K. O'Dowd |
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| Rok vydání: | 2009 |
| Předmět: |
Patch-Clamp Techniques
Physiology Action Potentials chemistry.chemical_compound physiology [Sense Organs] Calcium Channels N-Type physiology [Action Potentials] Drosophila Proteins Neurotransmitter cytology drug effects physiology [Brain] Cells Cultured metabolism [Parasympathetic Nervous System] Neurons Neurotransmitter Agents diagnostic use [Green Fluorescent Proteins] Voltage-dependent calcium channel General Neuroscience pharmacology [Neurotoxins] Life Sciences Brain Sense Organs Articles metabolism [Neurotransmitter Agents] Immunohistochemistry Electrophysiology physiology [Motor Activity] metabolism [Neurons] Excitatory postsynaptic potential Drosophila genetics physiology [Calcium Channels N-Type] Drosophila Protein physiology [Excitatory Postsynaptic Potentials] Green Fluorescent Proteins Neurotoxins Motor Activity Parasympathetic Nervous System Animals Patch clamp Excitatory Postsynaptic Potentials physiology [Drosophila Proteins] Microscopy Electron chemistry metabolism [Synapses] Synapses Cholinergic Calcium Channels Neuroscience physiology [Calcium Channels] |
| Zdroj: | Gu, Huaiyu; Jiang, Shaojuan Amy; Campusano, Jorge M; Iniguez, Jorge; Su, Hailing; Hoang, Andy An; et al.(2009). Cav2-type calcium channels encoded by cac regulate AP-independent neurotransmitter release at cholinergic synapses in adult Drosophila brain.. Journal of neurophysiology, 101(1), 42-453. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/97t4v64t |
| ISSN: | 1522-1598 0022-3077 |
| Popis: | Voltage-gated calcium channels containing α1 subunits encoded by Cav2 family genes are critical in regulating release of neurotransmitter at chemical synapses. In Drosophila, cac is the only Cav2-type gene. Cacophony (CAC) channels are localized in motor neuron terminals where they have been shown to mediate evoked, but not AP-independent, release of glutamate at the larval neuromuscular junction (NMJ). Cultured embryonic neurons also express CAC channels, but there is no information about the properties of CAC-mediated currents in adult brain nor how these channels regulate transmission in central neural circuits where fast excitatory synaptic transmission is predominantly cholinergic. Here we report that wild-type neurons cultured from late stage pupal brains and antennal lobe projection neurons (PNs) examined in adult brains, express calcium currents with two components: a slow-inactivating current sensitive to the spider toxin Plectreurys toxin II (PLTXII) and a fast-inactivating PLTXII-resistant component. CAC channels are the major contributors to the slow-inactivating PLTXII-sensitive current based on selective reduction of this component in hypomorphic cac mutants ( NT27 and TS3). Another characteristic of cac mutant neurons both in culture and in whole brain recordings is a reduced cholinergic miniature excitatory postsynaptic current frequency that is mimicked in wild-type neurons by acute application of PLTXII. These data demonstrate that cac encoded Cav2-type calcium channels regulate action potential (AP)-independent release of neurotransmitter at excitatory cholinergic synapses in the adult brain, a function not predicted from studies at the larval NMJ. |
| Databáze: | OpenAIRE |
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