AtPng1 knockout mutant of Arabidopsis thaliana shows a juvenile phenotype, morpho-functional changes, altered stress response and cell wall modifications
| Autor: | M. Della Mea, Giampiero Cai, Donatella Serafini-Fracassini, Luigi Parrotta, S. Del Duca, Iris Aloisi, Claudia Faleri |
|---|---|
| Přispěvatelé: | Serafini-Fracassini D., Della Mea M., Parrotta L., Faleri C., Cai G., Del Duca S., Aloisi I. |
| Rok vydání: | 2021 |
| Předmět: |
Polyamine
Chloroplasts Physiology Cellular differentiation Mutant A. thaliana Transglutaminase AtPng1p Differentiation Phenotype Abiotic stress Cell wall Polyamines Arabidopsis Plant Development Plant Science Biology medicine.disease_cause Chloroplast Cell wall Gene Knockout Techniques Polyamines Genetics medicine AtPng1p Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase Arabidopsis thaliana Mutation integumentary system Arabidopsis Proteins Abiotic stress Gene Knockout Technique Wild type food and beverages biology.organism_classification Transglutaminase Phenotype Cell biology Abiotic stre A. thaliana Differentiation Arabidopsi |
| Zdroj: | Plant Physiology and Biochemistry. 167:11-21 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2021.07.024 |
| Popis: | In order to ascertain the role of plant transglutaminases (TGase) in growth and abiotic stress response, the AtPng1 knock out (KO) line of A. thaliana has been analyzed during plant development and under heat and wound stress. Comparing wild type (WT) and KO lines a 58-kDa band was immunodetected by anti-AtPng1p antibody in the cell wall and chloroplasts only in the WT line. A residual TGase activity, not showing correlation with development nor stress response, was still present in the KO line. The KO line was less developed, with a juvenile phenotype characterized by fewer, smaller and less differentiated cells. Chloroplast TGase activity was insensitive to mutation. Data on stressed plants showed that (i) KO plants under heat stress were more juvenile compared to WT, (ii) different responses between WT and KO lines after wounding took place. TGase activity was not completely absent in the KO line, presenting high activity in the plastidial fraction. In general, the mutation affected A. thaliana growth and development, causing less differentiated cytological and anatomical features. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect