Measuring cell surface elasticity on enteroaggregative Escherichia coli wild type and dispersin mutant by AFM
| Autor: | David P. Allison, Sankar Venkataraman, Claretta J. Sullivan, James P. Nataro, Mitchel J. Doktycz, Melissa Beckmann, Jennifer L. Morrell-Falvey |
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| Rok vydání: | 2005 |
| Předmět: |
Surface Properties
Mutant medicine.disease_cause Microscopy Atomic Force Bacterial cell structure Microbiology Cell wall Intestinal mucosa Cell Wall medicine Escherichia coli Point Mutation Instrumentation biology Chemistry Escherichia coli Proteins Wild type biology.organism_classification Atomic and Molecular Physics and Optics Elasticity Electronic Optical and Magnetic Materials Culture Media Agar Biochemistry Enteroaggregative Escherichia coli Bacteria |
| Zdroj: | Ultramicroscopy. 106(8-9) |
| ISSN: | 0304-3991 |
| Popis: | Enteroaggregative Escherichia coli (EAEC) is pathogenic and produces severe diarrhea in humans. A mutant of EAEC that does not produce dispersin, a cell surface protein, is not pathogenic. It has been proposed that dispersin imparts a positive charge to the bacterial cell surface allowing the bacteria to colonize on the negatively charged intestinal mucosa. However, physical properties of the bacterial cell surface, such as rigidity, may be influenced by the presence of dispersin and may contribute to pathogenicity. Using the system developed in our laboratory for mounting and imaging bacterial cells by atomic force microscopy (AFM), in liquid, on gelatin coated mica surfaces, studies were initiated to measure cell surface elasticity. This was carried out in both wild type EAEC, that produces dispersin, and the mutant that does not produce dispersin. This was accomplished using AFM force-distance (FD) spectroscopy on the wild type and mutant grown in liquid or on solid medium. Images in liquid and in air of both the wild-type and mutant grown in liquid and on solid media are presented. This work represents an initial step in efforts to understand the pathogenic role of the dispersin protein in the wild-type bacteria. |
| Databáze: | OpenAIRE |
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