A Simple Whole-Blood Test for Detecting Antibodies to Human Immunodeficiency Virus
| Autor: | Stephen E. Wade, Patricia Castillo Torres, Anthony Burgess-Cassler, Willfried Schramm, Gustavo Barriga Angulo |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Microbiology (medical)
Fingerstick Clinical Biochemistry Immunology Human immunodeficiency virus (HIV) HIV Infections medicine.disease_cause Article Serology medicine Humans Immunology and Allergy Serologic Tests Seroconversion Whole blood medicine.diagnostic_test biology business.industry Virology Blot Immunoassay HIV-2 HIV-1 biology.protein Antibody business |
| Zdroj: | Clinical Diagnostic Laboratory Immunology. 5:263-265 |
| ISSN: | 1098-6588 1071-412X |
| DOI: | 10.1128/cdli.5.2.263-265.1998 |
| Popis: | We developed an immunochromatographic whole-blood test (WBT) which detects antibodies to human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) from fingerstick blood. The sensitivity and specificity of the WBT were 99.41% (1,018 confirmed positive patients) and 99.89% (941 uninfected patients), respectively (enzyme immunoassay [EIA] on serum or plasma as a reference). WBT performance was comparable to those of licensed EIAs and Western blotting, using 18 HIV-2 sera, 23 HIV-1 seroconversion panels, and a low-titer performance panel (in lieu of whole blood). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|