Maximizing confidence in a negative result: Quantitative sample adequacy control

Autor: Shaun Eintracht, Andreas I. Papadakis, Bruno Lamontagne, Ivan Brukner, Matthew Oughton, David Faucher, Alan Spatz
Rok vydání: 2020
Předmět:
0301 basic medicine
Oncology
medicine.medical_specialty
Sample (material)
030106 microbiology
Sample processing
Infection control
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Rapid detection
Specimen Handling
Laboratory
lcsh:Infectious and parasitic diseases
Nasal swab
03 medical and health sciences
0302 clinical medicine
Nasopharynx
Respiratory infection
Internal medicine
Influenza
Human
medicine
Humans
Mass Screening
lcsh:RC109-216
030212 general & internal medicine
False Negative Reactions
Protocol (science)
Pandemia
Cycle threshold
Routine screening
Diagnostic Tests
Routine
business.industry
lcsh:Public aspects of medicine
Public Health
Environmental and Occupational Health
lcsh:RA1-1270
Influenza a
General Medicine
qPCR
Infectious Diseases
Molecular Diagnostic Techniques
business
Zdroj: Journal of Infection and Public Health, Vol 13, Iss 7, Pp 991-993 (2020)
ISSN: 1876-0341
DOI: 10.1016/j.jiph.2020.01.307
Popis: Quantitative PCR (qPCR) is a leading screening tool, permitting rapid detection of pathogens and the maintenance of effective infection control programs. Unfortunately, qPCR assays frequently do not incorporate Sample Adequacy Control (SAC). A SAC controls for the quantity, quality and adequacy of the specimen. Without SAC, the confidence in a negative result remains questionable and the efficacy of screening is compromised. Ultimately, the exclusion of SAC from qPCR may result in false negative results. One should consider SAC to be an integral critical type of laboratory control; addressing diverse analytical problems, such as sample adequacy, sample processing and assay inhibition. Following distribution of cycle threshold values (Cq) of Influenza A positive results and Cq values of SAC, obtained from nasopharyngeal swabs, we showed that the confidence in a negative result cannot be guaranteed in the presence of a weak positive SAC signal (late Cq values). Herein, we explain why widespread inclusion of sample adequacy control in routine screening is blocked. A protocol and methods for SAC threshold establishment are offered.
Databáze: OpenAIRE
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