Expression of Magainin Antimicrobial Peptide Genes in the Developing Granular Glands of Xenopus Skin and Induction by Thyroid Hormone
| Autor: | Natale Tomassini, Dorothy Silver Reilly, Michael Zasloff |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Peptide Biosynthesis
Thyroid Hormones medicine.medical_specialty media_common.quotation_subject Molecular Sequence Data Xenopus Gene Expression Peptide In situ hybridization Xenopus Proteins Magainins Sebaceous Glands Xenopus laevis chemistry.chemical_compound Anti-Infective Agents Internal medicine Gene expression medicine Animals RNA Messenger Metamorphosis Molecular Biology Chromatography High Pressure Liquid media_common chemistry.chemical_classification Base Sequence biology Thyroid Metamorphosis Biological Magainin Cell Biology biology.organism_classification Immunohistochemistry Anti-Bacterial Agents Cell biology Endocrinology medicine.anatomical_structure Oligodeoxyribonucleotides chemistry Peptides Antimicrobial Cationic Peptides Developmental Biology Hormone |
| Zdroj: | Developmental Biology. 162:123-133 |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.1994.1072 |
| Popis: | The granular gland of amphibian skin is an epithelial derivative that first appears during metamorphosis. The granular glands of Xenopus laevis are an abundant source of magainins, a family of peptides with antimicrobial activity in vitro. We demonstrate the developmental expression and thyroid hormone induction of magainin and PGLa (peptide with aminoterminal glycine and carboxyterminal leucinamide), the two most abundant members of the magainin peptide family. Magainin and PGLa mRNA levels increase dramatically over the 2-day period between stage 58 and stage 60 and remain high during the remainder of metamorphosis. We used in situ hybridization to localize magainin and PGLa mRNAs to the developing granular gland. Magainin and PGLa peptides are also first detected during metamorphosis, as demonstrated by extraction of active peptides from stage 60 tadpoles and immunogold histochemical localization of magainin and PGLa to the granules of the skin granular gland. We demonstrate the premature induction of magainin and PGLa expression upon administration of exogenous thyroid hormone to stage 54 tadpoles. Further investigation of the developmental expression and induction of the magainin peptides will provide insight into their possible biological functions during Xenopus metamorphosis. |
| Databáze: | OpenAIRE |
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