Differential Expression of Virulence-Related Genes in Enterococcus faecalis in Response to Biological Cues in Serum and Urine
Autor: | Michael S. Gilmore, Brett D. Shepard |
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Jazyk: | angličtina |
Rok vydání: | 2002 |
Předmět: |
Immunology
Virulence Gene Expression Molecular Genomics Biology Microbiology Polymerase Chain Reaction Enterococcus faecalis law.invention law In vivo Gene expression medicine Humans RNA Messenger Gene Polymerase chain reaction Gram-Positive Bacterial Infections Gene Expression Profiling Sequence Analysis DNA medicine.disease biology.organism_classification Gene expression profiling RNA Bacterial Infectious Diseases Genes Bacterial Bacteremia Parasitology |
Popis: | Enterococci rank among leading causes of nosocomial bacteremia and urinary tract infection and are also a leading cause of community acquired subacute endocarditis. Limited evidence suggests that biological cues in serum and urine may play an important role in modulating enterococcal virulence at sites of infection. To determine the extent to which biological cues affect enterococcal virulence-associated gene expression, we used quantitative real-time PCR to compare mRNA levels in Enterococcus faecalis cultures grown in serum or urine to that achieved in laboratory medium. Both environment- and growth phase-specific variations were observed, demonstrating the occurrence of as-yet-uncharacterized mechanisms for control of gene expression in E. faecalis that may play an important role in vivo. |
Databáze: | OpenAIRE |
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