Influence of tetracyclines on human polymorphonuclear leukocyte function
| Autor: | G Hopen, C O Solberg, Sverre Sandberg, J Glette |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Neutrophils
Minocycline Oxytetracycline In Vitro Techniques Granulocyte Divalent law.invention Cell Movement law Cations medicine Humans Pharmacology (medical) Chelation Chemiluminescence Pharmacology Doxycycline chemistry.chemical_classification Chromatography Chemistry In vitro Glucose Spectrometry Fluorescence Infectious Diseases medicine.anatomical_structure Biochemistry Tetracyclines Luminescent Measurements Oxidation-Reduction Research Article medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 25:354-357 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.25.3.354 |
| Popis: | Low concentrations of oxytetracycline, doxycycline, or minocycline (less than 10 micrograms/ml) did not influence in vitro polymorphonuclear leukocyte random migration, chemiluminescence, or glucose oxidation. At high concentrations of doxycycline or minocycline (greater than 10 micrograms/ml), chemiluminescence and glucose oxidation were impaired. High concentrations of doxycycline also reduced random migration. Oxytetracycline did not influence these functions in concentrations up to 100 micrograms/ml. The inhibiting effect of doxycycline and minocycline was abolished when 4 mM Mg2+ was added to the reaction mixture, and 4 mM Ca2+ partly restored minocycline-inhibited polymorphonuclear leukocyte functions. This indicates that the major effect of tetracyclines on in vitro polymorphonuclear leukocyte functions is mediated by their divalent cation chelating effect and that the results of in vitro experiments are highly dependent on the concentration of divalent cations in the reaction mixtures. The difference between the tetracyclines may be due to differences in lipid solubility, with solubility being highest for minocycline and lowest for oxytetracycline, or to different divalent cation chelating ability. |
| Databáze: | OpenAIRE |
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