Repertoire of human natural anti-glycan immunoglobulins. Do we have auto-antibodies?
| Autor: | Marko Vuskovic, E. M. Rapoport, Carlo Unverzagt, Inna S. Popova, Margaret E. Huflejt, Polina Obukhova, Nicolai V. Bovin, Nadezhda Shilova, Maksim Navakouski |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Glycan
Molecular Sequence Data Population Protein Array Analysis Biophysics Immunoglobulins Models Biological Biochemistry Cohort Studies 03 medical and health sciences 0302 clinical medicine Glycolipid Blood serum Antigen Polysaccharides Humans education Glycomics Molecular Biology Autoantibodies 030304 developmental biology 0303 health sciences education.field_of_study biology Molecular biology 3. Good health Glycoproteomics Epitope mapping Carbohydrate Sequence 030220 oncology & carcinogenesis biology.protein Antibody Epitope Mapping Protein Binding |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1820:1373-1382 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/j.bbagen.2012.02.005 |
| Popis: | Background Profiling of donor's antibodies using glycan arrays demonstrated presence of antibodies capable of binding to > 100 mammalian glycans or their fragments. For example, relatively high binding to Galα1-4Galβ1-4GlcNAc (P 1 ), Galα1-4Galβ1-4Glc (P k ), Galβ1-3GlcNAc (Le c ), 4-O-SuGalβ1-4GlcNAc, and GalNAcα1-3GalNAc (Fs) was found in all tested individuals. Affinity isolation using hapten-specific chromatography in combination with epitope mapping revealed their glycotopes. Notably, a significant part of the antibodies was capable of recognizing a fragment of larger glycans, for example, -Galβ1-4Glc of glycolipids, or Fucα1-3GlcNAc motif of Le X /Le Y antigens. Their epitope specificity did not vary between different healthy individuals. Nominally, all the mentioned immunoglobulins could be classified as auto-antibodies. Methods In this work we re-evaluated results published earlier and analyzed new data to address the question why autologous antibodies found in healthy individuals do not cause severe auto-immune reactions. Results In all cases the presumably “auto” antibodies were found to bind short fragments “subtracted” from larger glycans whereas recognition of the same fragment in the context of the whole natural chain was completely abolished. Thus, in spite of numerous formally positive signals observed on the printed glycan array, we are yet unable to identify in blood serum of healthy individuals true auto-antibodies capable of binding carbohydrate chains in their naturally occurring form. General significance The identified natural anti-glycan antibodies were found to be specific, high-titer and population conservative immunoglobulins — all of this suggesting as yet unknown biological role(s) of the studied proteins. This article is part of a Special Issue entitled Glycoproteomics. |
| Databáze: | OpenAIRE |
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