Asymmetric localization of the cell division machinery during Bacillus subtilis sporulation
| Autor: | Kit Pogliano, Javier Lopez-Garrido, Joseph Sugie, Kanika Khanna, Elizabeth Villa |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Spores cell division Electron Microscope Tomography Time Factors Cell division Structural Biology and Molecular Biophysics Bacillus subtilis SpoIIE peptidoglycan FtsZ Bacterial cell structure chemistry.chemical_compound 0302 clinical medicine FtsA Fluorescence microscope B. subtilis structural biology Biology (General) Cytoskeleton Spores Bacterial Microbiology and Infectious Disease Microscopy General Neuroscience Bacterial General Medicine cryo-electron tomography Cell biology Medicine Research Article Signal Transduction QH301-705.5 Science infectious disease Biology General Biochemistry Genetics and Molecular Biology Fluorescence 03 medical and health sciences Bacterial Proteins Operon molecular biophysics General Immunology and Microbiology fungi Cryoelectron Microscopy microbiology Gene Expression Regulation Bacterial biology.organism_classification Spore Cytoskeletal Proteins 030104 developmental biology chemistry Microscopy Fluorescence Gene Expression Regulation biology.protein Peptidoglycan Biochemistry and Cell Biology 030217 neurology & neurosurgery |
| Zdroj: | eLife eLife, Vol 10 (2021) |
| Popis: | The Gram-positive bacterium Bacillus subtilis can divide via two modes. During vegetative growth, the division septum is formed at the midcell to produce two equal daughter cells. However, during sporulation, the division septum is formed closer to one pole to yield a smaller forespore and a larger mother cell. Using cryo-electron tomography, genetics and fluorescence microscopy, we found that the organization of the division machinery is different in the two septa. While FtsAZ filaments, the major orchestrators of bacterial cell division, are present uniformly around the leading edge of the invaginating vegetative septa, they are only present on the mother cell side of the invaginating sporulation septa. We provide evidence suggesting that the different distribution and number of FtsAZ filaments impact septal thickness, causing vegetative septa to be thicker than sporulation septa already during constriction. Finally, we show that a sporulation-specific protein, SpoIIE, regulates asymmetric divisome localization and septal thickness during sporulation. |
| Databáze: | OpenAIRE |
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